甘薯瘟两种不同致病型的初步研究

刘中华; 余华; 方树民; 许泳清; 邱永祥; 蔡南通

甘薯瘟两种不同致病型的初步研究

1.
福建省农业科学院作物研究所,福建福州,350013;
2.
福建省农业科学院中心实验室,福建福州 350003;
3.
福建农林大学,福建福州 350002

基金项目:

国家现代农业产业技术体系(CARS-11-B-10、nycytx-16-B-9-1);福建省科技计划重大专项(2008NZ001-5)

详细信息

作者简介:
蔡南通(1962-2011),男,研究员

中图分类号: S 435.311
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出版历程
- 收稿日期: 2011-07-02
- 修回日期: 2011-08-11
- 刊出日期: 2011-12-15

Preliminary Study on the Two Different Pathotypes of Ralsionia solanacearum

1.
Crop Research Institute, Fujian Academy of Agricultural Sciences,Fuzhou, Fujian 350013,China;
2.
Central Laboratory,Fujian Academy of Agricultural Sciences,Fuzhou, Fujian 350003,China;
3.
Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002,China

摘要

摘要: 为明确甘薯瘟Ⅰ型菌与Ⅱ型菌接种同一甘薯品种后具有不同致病力的原因,对2种菌系进行了形态学观察和基因组DNA多态性差异分析。研究结果如下:(1)用甘薯瘟Ⅰ型菌与Ⅱ型菌分别接种金山57,结果表明金山57对Ⅰ型菌表现高抗,对Ⅱ型菌表现高感;(2)400倍显微镜和10 000倍电镜下观察结果表明,2种菌均为杆状菌、有极生鞭毛,两端钝圆或尖圆,形态学结构无差别;(3)经16SrDNA扩增检测,确认所提取2个菌系的DNA为细菌DNA,运用RAPD分子标记方法对2个菌系的基因组DNA进行PCR扩增,10条RAPD引物共扩增出104条带,其中多态性条带有25条,多态性条带比率为24.04%,2个菌系基因组DNA之间存在明显多态性,基因组DNA之间的差异可能是导致其致病力显著不同的内在原因。
- 甘薯瘟 /
- 菌系 /
- 致病力 /
- 16SrDNA /
- 随机护增变态性 /
- 多态性
Abstract: In order to obtain a reasonable explanation of the phenomenon that there are different virulence between Ralstonia solanacearum typeⅠand typeⅡon the sweetpotato variety Jinshan57, morphological and genome DNA polymorphism were studied in this study.The results were as follows: 1,vaccination results shows jinshan57 high resistance to typeⅠand high sensitive to typeⅡ;2, There was no difference in morphological structure of the two strains based on microscopy and electron microscopy; 3, The DNA which were extracted from the two strains were bacterial 16S rDNA, Polymorphism was found between the two strains by analysis of genome DNA using RAPD markers, and the ratio of polymorphic bands up to 24.04%.The results showed that the genetic diversity maybe contribute to the different virulence between the typeⅠ and typeⅡ strains.
- Ralstonia Solanacearum /
- Strains /
- virulence /
- 16SrDNA /
- RAPD /
- polymorphic

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参考文献(7)

[1]
[2]
[3]
[4]	王庆容,李顺,宋培勇,等.2种改进的未知细菌DNA提取方法[J].安徽农业科学,2009,37(12):5381-5382,5607.
[5]
[6]	SALANOUBAT M S,GENIN F,ARTIGUENAVE,et al.Genome sequence of the plant pathogen Ralsionia solanacearum [J].Nature,2002,415:497-502.
[7]	JONAS D, ROSENBAUM A, WEYRICH S, et al.Enzyme-linked inomunoassay for detection of PCR-amplified DNA of legionellaein bronchoaveolar fluid[J]. J Clin Microbiol,1995,33(50):1247-1257.