Identification and Pathogenicity Test of the Pathogens Causing Soybean Root Rot in Fujian
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摘要: 为明确福建省大豆根腐病病原菌的种类及组成,于2007~2009年从漳州、厦门、龙岩等地大豆产区采集根腐病标样216份,从中分离到307株丝状病原真菌。根据其培养性状、形态学特征、代表性菌株的致病性测定及rDNA-ITS序列分析,将这些病原菌分为5种,分别为尖孢镰刀菌(Fusarium oxysporum,112株)、茄腐镰刀菌(Fusarium solani,74株)、大豆疫霉菌(Phytophthora sojae,79株)、立枯丝核菌(Rhizoctonia solani,24株)和瓜果腐霉菌(Pythium aphanidermatum,18株)。致病性测定结果表明,5种病原菌的致病力存在明显差异,其中大豆疫霉菌、尖孢镰刀菌和茄腐镰刀菌的致病性较强,发病率分别为100%、97.8%和95.6%,立枯丝核菌、瓜果腐霉菌则相对较弱。Abstract: Two hundred and sixteen soybean root rot samples were collected from Zhangzhou, Xiamen, Longyan city in Fujian province during 2007 to 2009 in order to determine the fungal pathogens that could cause soybean root rot, and three hundred and seven filamentous fungi strains were isolated from the samples. Five groups of pathogen were identified, which were Fusarium oxysporum (112 isolates), Fusarium solani (74 isolates), Phytophthora sojae (79 isolates), Rhizoctonia solani (24 isolates),Pythium aphanidermatum (18 isolates) according to the cultural characteristics, morphology, pathogenicity and the rDNA-ITS sequences of the isolates. The results of the pathogenicity of these pathogens revealed that P. sojae, F. oxysporum and F. solani isolates showed strong pathogenicity to soybean roots by artifical infection, leading to disease incidences by 100%、97.8% and 95.6%, respectively, but R. solani and P. aphanidermatum isolates showed weak pathogenicity.
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Key words:
- soybean root rot /
- pathogen identification /
- ITS /
- pathogenicity
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