摘要:
以3个不同花色蝴蝶兰V31、夕阳红和大红花品种的丛生芽为试材,研究了植物生长调节剂对试管苗增殖与生根的影响。结果表明:3个品种在增殖过程中其增殖系数均随BA浓度的增加呈现先增后减的变化趋势,一定浓度的NAA促进蝴蝶兰生根,但是在丛生芽增殖与生根方面,不同品种之间存在一定程度的差异性。适宜于V31品种丛生芽增殖的培养基为基本培养基中添加5.0 mgL-1BA,培养50d增殖系数达到5.24,在基本培养基中添加0.5 mgL-1NAA培养70 d生根效果最佳;适宜于夕阳红品种增殖的培养基为基本培养基添加7.5 mgL-1BA,增殖系数为2.04,添加0.5 mgL-1NAA的生根培养基中其生根数、根长与叶片数均最佳;大红花品种则在基本培养基中添加10.0 mgL-1BA的其增殖效果最好,增殖系数为2.25,基本培养基中添加1.0 mgL-1NAA促进生根。
关键词:
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蝴蝶兰 /
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品种 /
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丛生芽 /
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增殖 /
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生根
Abstract:
Three Phalaenopsis cultivars, i.e., V31, Sunset Red and Dahonghua, were used for the experiment. Effects of plant growth regulators on the clusterd bud proliferation and rooting of the cultivars were studied. The results showed that in addition to the increase of BA concentration, the bud proliferation rate of the 3 cultivars increased initially, and then, decreased. Optimal NAA concentration in the culture medium could help improve rooting. However, its effects on bud prooliferation and rooting differed with cultivars. For V31, the proliferation increased on the basal medium containing 5.0 mgL-1BA (after 50 d of culture, the proliferation rate was 5.24), while the root growth, on the basal medium with addition of 0.5 mgL-1 NAA. The optimal medium for the Sunset Red was with 7.5 mgL-1 BA to reach a proliferation rate of 2.04 and 0.5 mgL-1 NAA to achieve an effective root induction. For Dahonghua, the proliferation was best improved by the addition of 10.0 mgL-1 BA (the rate was 2.25), and the root growth was best with addition of 1.0 mgL-1 NAA in the medium.
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