茶树不同外植体组织培养与试管苗保存研究初探

基金项目:

福建省属公益类科研院所基本科研专项(2010R1014-4)

A preliminary study on explant tissue culture and in vitro plantlet preservation for tea

1.
Tea Research Institute, Fujian Academy of Agricultural Science, Fu'an, Fujian 355000, China

摘要: 选取福鼎大白茶的种子、子叶、叶片、根、短穗作为外植体,进行愈伤组织诱导培养、愈伤组织继代培养和愈伤组织分化培养,结果表明:(1)叶片是诱导愈伤组织最好的外植体,适合的诱导培养基为N6+2,4-D(2.5mgL-1);(2)愈伤组织通过多次继代培养扩大繁殖,可获得充足的愈伤组织;(3)愈伤组织在20种分化培养基中分化生长,绝大多数愈伤能长出绿色小突起,有一部分愈伤能分化出根。
- 茶树 /
- 愈伤组织诱导培养 /
- 愈伤组织继代培养 /
- 愈伤组织分化培养 /
- 试管苗
Abstract: The callus induction,subculture and regeneration of tea explants of seeds,cotyledons,leaves,roots and short stems for Camellia.sinensis cv.Fudingdabai were conducted to determine material and means for preserving tea cultivars.The results showed that(a) the tea leaves were the best explant for inducing calli using N6+2,4-D at the concentration of 2.0 mgL-1 as the induction medium;(b) a great deal of calli could be obtained by application of the multiple-subculture technique;and(c) the color of most calli were green on the 20 regeneration media tested,however,roots could be regenerated from the calli only on some of the media.
- tea(Camellia sinensis) /
- callus induction /
- callus subculture /
- callus regeneration /
- test-tube plantlet

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