Isolation and gene sequence analysis of PRRSV-FJ07A strain's structural proteins
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摘要: 从福建某猪场发生疑似"高热病"死亡的小猪病料中分离到1株病毒,该病毒在Marc-145细胞上增殖致细胞病变。对分离病毒的TCID50、动物回归试验、RT-PCR鉴定及对病毒株的结构蛋白基因进行了测定与分析。结果表明:分离毒株为美洲型猪繁殖与呼吸综合征病毒(命名为PRRSV-FJ07A),TCID50为10-6.5mL-1,对35日仔猪具有致病性,结构蛋白基因与美洲型代表株VR-2332核苷酸同源性为89.2%~95.8%,氨基酸同源性为88.1%~96.2%;与国内PRRSV-HUN4株的核苷酸同源性为90.1%~99.7%,氨基酸同源性为88.3%~100.0%;与欧洲代表株LV的核苷酸同源性为63.7%~70.0%,氨基酸同源性为59.6%~78.9%。PRRSV-FJ07A序列已登陆Genbank,登录号为GI:283100314。
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关键词:
- 繁殖与呼吸综合征病毒 /
- 分离 /
- 序列分析 /
- 猪
Abstract: PRRSV-FJ07A strain was isolated and identified.The virus was tested on the Marc-145 cell and subjected to RT-PCR,TCID50,gene sequencing of structural protein and an animal challenge test.The results showed that PRRSV-FJ07A strain belonged to the family of the North American genotype of PRRSV.Its TCID50 was 10-6.5.ml-1.Compared with VR-2332 strain,the homogeneities of nucleotide and amino acid were 89.2%-95.8% and 88.1%-96.2%,respectively,90.1%-99.7% and 88.3%-100.0%,respectively,compared with HUN4 strain,and 63.7%-70.0% and 59.6%-78.9%,respectively,compared to and LV strain.After 3 to 7 days PRRSV-FJ07A innoculation,the 35-day-old piglets showed negative serum antibody of PRRSV and appeared with clinical syndromes of PRRSV infection.The gene sequence of PRRSV-FJ07A has been submitted to Genbank issued with the number of GI: 283100314.-
Key words:
- PRRSV /
- isolation /
- gene sequence analysis /
- swine
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[1] WELCH S K,JOLIER,PEARCE D S,et al.Construction and evaluation of genetically engineered replication-defective porcine reproductive and respiratory syndrome virus vaccine candidates[J].Vet Immunol Immunopathol,2004,102 (3):277-290.
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