花生离体培养条件下不同外植体对青枯菌粗毒素的抗性反应

Resistance to crude R.solanacearum toxin of in vitro cultured peanut explants

1.
R & D Center Fujian Xinrixian Group Co., Ltd., Fuzhou, Fujian 350002, China;
2.
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
3.
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要: 离体培养条件下花生不同外植体对青枯菌粗毒素抗性反应的测定结果表明,涂布法和培养基混毒素法均可初步测定出不同花生品种抗性程度。粗毒素对花生愈伤组织诱导率的测定结果表明,随着粗毒素浓度的提高,花生叶片的出愈率降低,但品种之间差异不明显。对花生愈伤组织的褐变率的测定表明,感病材料愈伤组织较为敏感,处理后较早出现褐变、崩溃等较重的受害症状,而抗病材料的症状出现较慢、较轻,花生愈伤组织的离体抗性与田间呈相同的趋势。
- 花生 /
- 品种 /
- 青枯菌 /
- 粗毒素 /
- 抗性鉴定
Abstract: Reaction of the peanut explants cultured in vitro to crude R.solanacearum toxin was studied.The results showed that the plant's resistance to the toxin could be determined by either smearing the toxin on the plant or mixing the toxin in the medium.It appeared that the percentage of callus formation on peanut leaf decreased as the toxin concentration increased,though not statistically significant among the samples.Based on the appearance of brown callus,the callus from the susceptible genotypes were more sensitive,showing greater browning,collapsing and other symptoms of damage,at earlier stage after treatment than the resistant plants.The in vitro observations seemed to parallel those in the field.
- Peanut /
- varieties /
- Ralstonia solanacearum /
- crude toxin /
- resistance

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