On breeding Dendrobium candidum Wall.ex Lindl
-
摘要: 以福建铁皮石斛种子为材料,开展种子萌芽、壮苗生根适宜培养基配方试验研究,并对组培苗的移栽基质进行筛选。结果表明:以MS+蔗糖30gL-1+琼脂7gL-1为培养基,种子萌芽快,萌芽率高;1/4MS+蔗糖40gL-1+马铃薯提取液100mgL-1+琼脂7gL-1是壮苗生根的最佳培养基;木屑:泥炭(1:2)的基质,组培苗移栽成活率、平均新芽数及大于3cm平均新芽数等3项指标最好。Abstract: On an appropriate medium,Fujian's Dendrobium candidum Wall.ex Lindl was studied for its seed germination,plantlet strength,rooting and transplantation.The results indicated that the seeds germinated well and quickly on the medium consisting of MS +sucrose 30 gL-1 +agar 7 gL-1.To strengthen the plantlet and encourage root formation,the best formulation was found to be 1/4MS +sucrose 40gL-1 +potato extract 100 mgL-1+agar 7gL-1.The transplanting survival rate,average number of new buds and average number of new buds that were longer than 3 cm could best be obtained when the mixture of sawdust and peat in the ratio of 1:2 was used for the plantlet transplantation.
-
Key words:
- Dendrobium candidum Wall.ex Lindl /
- tissue culture /
- medium
-
[1] 何铁光,杨丽涛,李杨瑞,等.铁皮石斛原球茎多糖DCPP1a1对氧自由基和脂质过氧化的影响[J].天然产物研究与开发,2007,19:410-414. [2] 邵华,张玲琪,李俊梅,等.铁皮石斛研究进展[J].中草药,2004,35(1):109-112. [3] 鲍顺淑,贺冬仙,郭顺星.可控环境下光照时间对铁皮石斛组培苗生长发育的影响[J].中国农业科技导报,2007,9(6):90-94. [4] 唐启义,冯明光.实用统计分析及其DPS数据处理系统[M].北京:科学出版社,2002:43-71,94-99. [5] 李进进,廖俊杰,许继勇,等.铁皮石斛试管苗栽培技术研究[J].中药材,2006,29(11):1133-1134. -
点击查看大图
计量
- 文章访问数: 4772
- HTML全文浏览量: 149
- PDF下载量: 847
- 被引次数: 0
下载:
