应用ISSR技术分析苦瓜种质资源的多态性

Determination of Momordica's germplasm diversity by ISSR

摘要: 运用ISSR分子标记,对48个苦瓜样品的遗传多样性进行研究。从96条随机引物中筛选出14条ISSR引物,共扩增出181条带,其中多态性谱带为113条,多态性比率为60.32%。聚类分析结果表明:48个苦瓜品种之间的遗传相似系数在0.685～0.865。

Abstract: The inter-simple sequence repeat(ISSR) method was applied to assess the genetic variations in 48 Momordica cultivars.From 96 ISSR primers 14 were employed on 48 samples.Out of 181 amplified bands,113 were found to be polymorphic(60.32%).The clustering analysis of ISSR results indicated that the genetic similarity indices were between 0.685 and 0.865.Thus,the genetic diversity,similarity and relationships among the cultivars were revealed providing a theoretical foundation for Momordica breeding in the future.