Isolation and activity of antibacterial compounds in Pleurotus sajor-caju Fermentation Broth
-
摘要: 以发酵培养的凤尾菇发酵液为材料,浓缩后经乙酸乙酯和正丁醇分级萃取,采用薄层层析和硅胶柱层析分离得到数个组分,研究了各组分对供试细菌的抗菌活性,并对部分活性组分进行GC-MS化学成分分析。结果表明:凤尾菇发酵液乙酸乙酯萃取物硅胶柱洗脱组分Epsc1、2及正丁醇萃取物硅胶柱洗脱组Npsc1、2具有抗菌活性,其中组分Npsc1对金黄色葡萄球菌抑制作用最强。Abstract: The enriched fermentation broth of Pleurotus sajor-caju was extracted with ethyl acetate and followed by n-butanol.Several compounds were obtained from the extract by using thin layer chromatography and silica gel column chromatography.The isolated compounds were tested for their antibacterial activities.The active substances were further analyzed using GC-MS.Among them,Epsc1 and 2 and Npsc1 and 2 showed antibacterial activities,and Npsc1 had the strongest inhibitory effect on Staphylococeu aureus.
-
Key words:
- Pleurotus sajor-caju /
- fermentation broth /
- antibacteria /
- active component
-
[1] ASGHER M,BHATTi H N,ASHRAF M,et al.Recent developments in biodegradation of industrial pollutants by white rot fungi and their enzyme system[J].Biodegradation,2008,19:771-783. [2] CONFORTIN F G,MARCHETTO R,BETTIN F,et al.Production of Pleurotus sajor-caju strain PS2001 biomassin submerged culture[J].J Ind Microbiol Biotechnol,2008,35:1149-1155. [3] 曾志恒,郑怡,刘艳如,等.凤尾菇深层发酵液抗细菌抗真菌活性的研究[J].福建师范大学:自然科学版,2010,26 (3):72-77. [4] 林陈强,林戎斌,蔡海松,等.虎奶菇抑菌物质与食品防腐剂抑菌活性的比较[J].食用菌学报,2007,14(3):62-66. [5] 李作平,章克昌.灵芝发酵菌丝体中灵芝酸的分离纯化及生物活性检测[J].天然产物研究与开发,1999,11(4):67-70.
点击查看大图
计量
- 文章访问数: 2297
- HTML全文浏览量: 99
- PDF下载量: 746
- 被引次数: 0