提高迟钝爱德华氏菌细胞生物量的发酵条件研究

提高迟钝爱德华氏菌细胞生物量的发酵条件研究

1.
福州大学生物科学与工程学院, 福建, 福州 350108;
2.
福建省农业科学院生物技术研究所, 福建, 福州 350003

基金项目:

农业部公益项目(nyhyzx07-043-15);国家863项目(2008AA92501);福建省科技计划项目(2009N2003)

详细信息

中图分类号: Q93
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出版历程
- 收稿日期: 2010-08-27
- 修回日期: 2010-10-09
- 刊出日期: 2010-10-15

Optimization of Edwardsiella tarda Fermentation to increase biomass

1.
College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China;
2.
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要

摘要: 以胰大豆蛋白胨培养基(TSB)为基础培养基,采用单因素和正交试验方法优化提高鳗源迟钝爱德华氏菌ETY细胞生物量的培养基与培养条件。通过试验,获得迟钝爱德华氏菌ETY的优化培养基配方为1000mL水加入葡萄糖3.0g、胰蛋白胨10.0g、鱼粉蛋白胨10.0g、磷酸氢二钾2.5g、氯化钠5.0g;培养条件优化结果为接种量8%、温度25℃、pH 7.5、转速200rmin-1。迟钝爱德华氏菌ETY在此条件下培养20h,细胞生物量达1.575×1010 cfumL-1,较优化前培养的细胞生物量5.200×109 cfumL-1提高3倍。每吨液体培养基的成本较优化前下降41.36%。
- 迟钝爱德华氏菌 /
- 提高 /
- 细胞生物量 /
- 发酵 /
- 条件优化
Abstract: Using tryptone soya broth medium,the culture conditions of Edwardsiella tarda(ETY) were optimized by a single factor and an orthogonal test to enhance the cell concentration.The results showed that the optimized medium contained 3.0 g of glucose,10.0 g of tryptone,10.0 g of peptone,2.50 g of K2HPO4 and 5.0 g of NaCl per liter.And,the most favorable fermentation conditions were at 25℃,with an initial pH of 7.5,on a stirring speed of 200 rmin-1 and with the inoculating ratio of 8%.Compared with the control,the cell concentration of ETY could be improved from 5.200×109cfumL-1 to 1.575×1010cfumL-1,i.e.,3 times increase.At the same time,the cost of liquid medium was reduced by 41.36% compared to the conventional formulation.
- Edwardsiella tarda /
- cell concentration /
- fermentation /
- optimization /