百合试管鳞茎诱导及膨大技术的研究

张洁; 蔡宣梅; 林真; 郭文杰; 方少忠

百合试管鳞茎诱导及膨大技术的研究

1.
福建省农业科学院生物技术研究所, 福建, 福州, 350003

基金项目:

福建省属公益类科研院所基本科研专项(2009R10035-7)

详细信息

通讯作者:
方少忠(1969-),男,硕士,副研究员,从事园艺产品研究(E-mail:Fangsha6988@yahoo.com.cn)

中图分类号: S682.29
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- 被引次数: 0
出版历程
- 收稿日期: 2010-03-15
- 修回日期: 2010-04-21
- 刊出日期: 2010-06-15

Formation and enlargement of lily bulblet in test tube

1.
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要

摘要: 以东方百合"Sorbonne"试管苗为材料,研究了蔗糖浓度、大量元素、培养基状态、多效唑和水杨酸浓度对百合试管鳞茎诱导及膨大的影响.结果表明,蔗糖浓度为60gL-1时新增鳞茎数最多,鳞茎形成率达75%,蔗糖浓度120gL-1处理最有利于鳞茎膨大;高浓度的大量元素有利于鳞茎的膨大,而不利于鳞茎的诱导,3MS时形成的鳞茎最大,但鳞茎形成率则下降到14.6%;液体培养基对鳞茎鲜重影响显著;多效唑浓度10mgL-1处理能促进鳞茎的诱导和膨大;水杨酸处理则能显著提高试管鳞茎的数量.
- 百合 /
- 试管鳞茎 /
- 组织培养
Abstract: The effects of sugar concentration,macroelement,paclobutrazol,salicylic acid and culture condition on bulblet formation and enlargement were studied using plantlet of Oriental Lily,'Sorbonne',in test tubes.The results indicated that,at 60 gL-1 sucrose concentration,the most numbers of the bulblets was formed at the rate of 75%.At 120 gL-1 sucrose concentration,the enlargement of micro-bulb was most significant.High macroelement concentrations tended to favor the enlargement as well,but discouraged formation of the bulblet in the test tubes.The 3MS treatment produced the largest bulblets,but the rate of bulblet formation dropped to 14.6%.The addition of 10 mgL-1 paclobutrazol could aid the bulblet formation and enlargement in the test tubes.And,the application of salicylic acid significantly increased the number of the micro-bulbs formed.
- lily /
- bulblet in tube /
- tissue culture

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参考文献(3)

[1]	王家福,陈振光.百合快速繁殖条件的优化[J].福建农业大学学报,1999,28(2):152-156.
[2]	李春香,杨军,王树才,等.水杨酸在大蒜鳞茎膨大中的作用[J].园艺学报,2000,27(3):220-222.
[3]	NIIMI Y,MISAKI Y,NAKANOM.Production of commercial bulbs of Lilium rubellum baker:Changes in carbohydrates in bulblets and sugars of liquid medium during their culture[J].Journal of the Japanese Society for Horticultural Science,2000,69(2):161-165.