Proliferation and maturation of somatic embryos of slash pine
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摘要: 以湿地松未成熟合子胚为试验材料,诱导获得胚性胚柄细胞团(Embryogenic Suspensor Mass-ESM),通过培养基的优化,开展湿地松ESM维持与增殖及体胚成熟培养的研究。研究结果表明:基本培养基与植物生长调节剂组合影响ESM的增殖速率及质量,DCR+NAA1.0mgL-1+6-BA0.32mgL-1+KT0.30mgL-1为湿地松ESM的最佳增殖培养基;在无植物生长调节剂培养基上的预培养是湿地松体胚发育、成熟的必要程序,ABA、PEG、AC和高浓度蔗糖的组合进一步促进了体胚的发育成熟,体胚在培养基LP+ABA50mgL-1+PEG600020%+6%蔗糖+AC2.5gL-1上获得了近子叶期体胚,但未能获得完全成熟的体胚。Abstract: Embryogenic suspensor mass (ESM) of slash pine was induced with the non-mature zygotic embryos as explants. The maintenance,proliferation and maturation of ESM culture were studied with medium optimization. The results indicated that (a) the basic medium in combination of plant growth regulation (PGR) agents affected the proliferation rate and the quality of somatic embryos (SEs); (b) the optimized medium contained DCR+1.0 mg NAAL-1+0.32 mg 6-BAL-1+0.30 mg KTL-1; (c) in medium without PGR,the pretreatment was necessary for the development and maturation of SEs of the slash pine; (d) the combination of ABA,PEG,AC and a high concentration of sucrose improved the maturation of SEs; and (e) the SEs developed into stage 5 on medium of LP+50 mg ABAL-1+20% PEG6000+6% sucrose+2.5 g ACL-1,but no mature embryo was obtained.
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Key words:
- Pinus elliottii /
- ESM /
- maintenance of SEs /
- proliferation of SEs /
- maturation of SEs
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[1] ARYA S,KALIA R K,ARYA I D.Induction of somatic embryogenesis in pinus roxburghii Sarg[J].Plant Cell Reports,2000,19:775-780. [2] 吕守芳,张守攻,齐力旺,等.日本落叶松体细胞胚胎发生的研究[J].林业科学,2005,41(2):48-52.
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