摘要:
以芦笋品种"Gijnlim"为试验材料,研究花药预处理、培养基附加物、培养方式等对愈伤组织类型、诱导率和绿苗分化率的影响,探讨愈伤组织类型与染色体倍性比例间的关系。结果表明,芦笋花药在4℃低温下冷藏5～7d后接种于含有NAA2.0mgL-1、6-BA1.0mgL-1及6%蔗糖的1/2MS培养基上对愈伤组织的诱导最为有利;接种后进行32℃的高温处理5d,有利于提高愈伤组织的诱导率;培养基中加入水解酪蛋白(CH)300mgL-1有利于提高愈伤组织的诱导率;愈伤组织的类型和日龄影响绿苗的分化率,致密型愈伤组织的分化率高于松散型愈伤组织,30～40d日龄的愈伤组织分化率最高。镜检结果表明,致密型愈伤组织分化的单倍体多于松散型。
Abstract:
Experiments were conducted to study the callus inducement and green shoot differentiation of the anther of the breed "Gijnlim",Asparagus officinalis L. The results showed that the optimum asparagus callus induction could be obtained by inoculation on a 1/2 MS culture medium containing 2.0 mg NAAL-1,1.0 mg 6-BAL-1and 6% sucrose after storage at 4℃ for 5-7 days. Moreover,a higher induction rate could be accomplished with a 32℃ incubation for 5 days following the inoculation. Supplementation with 300 mg hydrolyzed caseinL-1in the MS medium also benefited the callus induction. Callus type and age affected the regeneration rate. The regeneration rate of the callus of the dense form was higher than that of the loose form. On the different callus ages (i.e.,10-50 days),30-to 40-day-olds had the highest green shoots regeneration rate. The microscopic examination showed that,among the green shoots from the loose callus,5.7% of the plants were haploids,65.1% diploids,11.9% tetraploids and 17.3% aneuploids; while among the green shoots from the dense callus,28.4% were haploids,45.3% diploids,9.6% tetraploids and 16.7% aneuploids; and,the haploids from the dense callus were more than those from the loose callus.
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