新铁炮百合花器官组织培养研究

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基金项目:

福建省科技计划重点项目(2008N0071);福建省星火科技计划项目(2009S0107);福建省林业科技重大专项(2006NZ0001-3);国家科技支撑计划项目(2007BAD07B03)

Organ tissue culture of Lilium formolongi

1.
Crop Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Fujian Engineering Research Center for Characteristic Floriculture, Fuzhou, Fujian 350013, China

摘要: 以新铁炮百合的花梗、花托、花瓣、花丝和花柱(去除柱头)为外植体,进行离体培养及快速繁殖研究。结果表明,5种花器官均能直接诱导产生不定芽,其诱导能力大小为花托花梗花丝花瓣、花柱。诱导不定芽的适宜培养基为:MS+2.0mgL-16-BA+0.2mgL-1NAA;不定芽增殖的适宜培养基为MS+2.0mgL-16-BA+1.0mgL-1KT+0.2mgL-1NAA;生根的适宜培养基为1/2MS+0.03mgL-1NAA;适合新铁炮百合小鳞茎种植的基质为1/4园土+1/4泥炭土+1/2锯末。
- 新铁炮百合 /
- 花器官 /
- 组织培养
Abstract: The floral organ culture and rapid propagation of Lilium formolongi was studied by using its pedicel,receptacle,petal,filament and style for explants. The results showed that (a) adventitious buds could be induced directly from all of the 5 floral organs; (b) the appropriate medium was formulated with MS+2.0 mg 6-BAL-1+0.2 mg NAAL-1; (c) the order of inducing ability of the organs was:receptaclepedicelfilamentpetal or style; (d) the optimum medium formulation for adventitious buds proliferation was:MS+2.0 mg 6-BAL-1+1.0mg KTL-1+0.2 mg NAAL-1; (e) the appropriate medium for in vitro root and bulb formations was:1/2MS+0.03 mg NAAL-1; and (f) the most efficient and low-cost medium for transplantation was:1/4 (v/V) garden mould+1/4 (v/V) peat mould+1/2 (v/V) sawdust.
- Lilium formolongi /
- floral organ /
- tissue culture