基于ITS序列探讨南瓜属植物系统发育

吴宇芬; 周先治; 陈晟; 陈阳; 赵依杰

基于ITS序列探讨南瓜属植物系统发育

1.
福建省农业科学院农业生物资源研究所, 福建, 福州, 350003;
2.
福建省福州市农业科学研究所, 福建, 福州, 350018

基金项目:

福建省科技计划重点项目(2007I0037);福建省自然科学基金项目(2009J01069)

详细信息

通讯作者:
周先治(1976- ),男,助理研究员,博士,主要从事生物资源研究(E-mail:xianzhizhou@126.com)

中图分类号: S642
计量
- 文章访问数: 2674
- HTML全文浏览量: 144
- PDF下载量: 594
- 被引次数: 0
出版历程
- 收稿日期: 2010-01-05
- 修回日期: 2010-04-01
- 刊出日期: 2010-04-15

Phylogeny of Cucurbita Based on ITS sequence data

1.
Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2.
Fuzhou Institute of Agricuhural Sciences, Fuzhou, Fujian 350018, China

摘要

摘要: 基于ITS序列对南瓜属3个种(中国南瓜、美洲南瓜和黑籽南瓜)的系统发育进行了研究。结果表明,ITS1全长188～191bp,ITS2全长251～263bp;南瓜ITS1序列的变异位点数为33,占ITS1全序列的17.55%,ITS1序列的信息位点数为24,占ITS1全序列的12.77%;南瓜ITS2序列的变异位点数为45个,占ITS2全序列的17.93%,ITS2的信息位点数为31个,占ITS2全序列的12.35%;ITS1的G+C含量变化范围为51.60%～54.50%,ITS2的G+C含量变化范围为53.78%～56.98%;基于ITS1和ITS2分别构建的系统树可以看出,中国南瓜和美洲南瓜的亲缘关系较近,与黑籽南瓜的亲缘关系较远。
- rDNA /
- 内转录间隔区 /
- 南瓜属 /
- 系统发育
Abstract: The relationships and differences among the species,Cucurbita,pepo and ficifofia (Cucurbitaceae:Cucurbita),were examined using data from the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA. The ITS sequences were obtained from Cucurbita,pepo and ficifofia 3 species. The results showed that,within Cucurbita,ITS1 was 188-191bp (C. Cucurbita,C. pepo and C. ficifofia) and ITS2 ranged from 251-263bp (C. Cucurbita,C. pepo and C. ficifofia). There were 33 variable positions (17.55%) and 24 informative sites (12.77%) within Cucurbita found in the ITS1 sequences,while 45 variable positions (17.93%) and 31 informative sites (12.35%) found in the ITS2 sequences.The G+C content of ITS1 was 51.60% to 54.50% and that of ITS2 ranged from 53.78% to 56.98%.The resulting trees from the ITS1 and ITS2 sequences showed that the relationship between C. Cucurbita and C. pepo was closer than that between C. Cucurbita and C. ficifofia.
- Ribosomal DNA (rDNA) /
- Internal transcribed spacer (ITS ) /
- Cucurbita /
- phylogeny

HTML全文

参考文献(4)

[1]	赵福宽,李海英,赵晓萌.南瓜种质资源亲缘关系的RAPD分析[J].分子植物育种,2006,4 (3):45-50.
[2]	张天明,屈东玉,王长林,等.南瓜属4个栽培种亲缘关系的AFLP分析[J].中国蔬菜,2006(1):11-14.
[3]	HSIAO C,CHATTERTON N J,ASAY K H,et al.Phylogenetic relationships of the monogenomic species of the wheat tribe,Triticeae(Poaceae),inferred from nuclear rDNA (internal transcribed spacer) sequences[J].Genome,1995,38 (2):211-223.
[4]	戴小军,梁满中,陈良碧.栽培稻种内核糖体基因的ITS序列比较研究[J].作物学报,2007,33 (11):1874-1878.