呼吸道综合症病猪群病原检测与分析

叶耀辉; 吴南洋; 黄梅清; 郑敏; 雷松波; 张世忠

呼吸道综合症病猪群病原检测与分析

1.
福建省宁德市农业科学研究所, 福建, 福安, 355017;
2.
福建省农业科学院畜牧兽医研究所, 福建, 福州, 350013

基金项目:

福建省科技计划项目(2008N0113)

详细信息

通讯作者:
吴南洋(1963- ),男,高级兽医师,主要从事规模化猪场疫病控制研究

中图分类号: S855.3
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- 被引次数: 0
出版历程
- 收稿日期: 2010-01-05
- 修回日期: 2010-03-16
- 刊出日期: 2010-04-15

Pathogenic detection and identification of swine respiratory syndrome

1.
Ningde Agricultural Science Research Institute of Fijian Province, Fu'an, Fujian 355017, China;
2.
Institute of Animal Husbandry and Veterinary Medicine, Fijian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 应用PCR技术和免疫荧光试验对福建省某猪场以呼吸道症状为主的发病猪群进行多重病原检测和人工感染病猪肺脏淋巴结等器官的病理组织学观察。结果显示:病猪肺脏和淋巴结等病料匀浆的RT-PCR检测为PRRSV变异株阳性,但PRV、CSFV、PCV2及SIV均为阴性;免疫荧光试验结果与RT-PCR一致;将阳性病料接种Marc-145分离到1株病毒,人工感染断奶仔猪能复制出与临床自然病猪相同的临床症状和病理变化,并能回收到病毒;病理组织学观察显示人工感染濒死猪肺脏呈间质性肺炎病变。综合分析上述结果初步表明该猪群暴发的呼吸道疾病主要是由高致病性猪繁殖与呼吸综合征病毒(PRRSV)变异型引起。
- 呼吸道疾病 /
- 猪群 /
- 高致病性猪繁殖与呼吸综合征病毒(PRRSV) /
- 病原检测 /
- 变异型
Abstract: The study detected various kinds of pathogens in pigs suffering from respiratory syndrome by PCR technique and immunofluorescent test. The RT-PCR results showed that the tissue samples of lung and lymph nodes from diseased pigs were positive for PRRSV variant but negative for PRV,CSFV,PCV2 or SIV. A virus strains were also isolated from positive samples by inoculating it onto Marc-145. The 30 days-old piglets inoculated with the isolates showed the same symptoms as appeared naturally on the diseased pigs in the clinic. The virus was recovered from and the interstitial Pneumonia observed in the diseased pigs. Thus,it was concluded that the respiratory disease outbreak in the swine farm was caused by a PRRSV variant.
- respiratory disease /
- swine /
- porcine reproductive and respiratory syndrome virus (PRRSV) /
- pathogen detection /
- variant

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参考文献(2)

[1]	车勇良,王隆柏,魏宏,等.PCR技术在猪圆环病毒2型检测中的应用[J].动物医学进展,2006,27(6):70-80,102.
[2]	郝晓芳,周艳君,田志军,等.高致病性猪繁殖与呼吸综合征病毒RT-PCR鉴别诊断方法的建立[J].中国预防兽医学报,2007,29(9):704-709.