摘要:
以15年实生木麻黄根系水浸液作为他感作用物,采用水培方法研究木麻黄自身他感作用物对木麻黄苗木活性氧代谢的影响.结果表明,在木麻黄自身他感作用下,木麻黄苗木叶片活性氧代谢失衡,活性氧大量积累.具体表现在:细胞膜透性增强,膜的结构和功能受到破坏,电解质增强,内溶物外渗增加;丙二醛(MDA)含量增加,膜质过氧化程度加剧;叶细胞中超氧阴离子自由基O2-产生的速率和H2O2含量增加,活性氧产生和清除平衡被打破,细胞内活性氧大量累积;活性氧酶促消除系统失衡,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱苷肽还原酶(GR)和过氧化物酶(POD)活性下降,细胞清除O2-和H2O2的能力减弱.
Abstract:
Effect of self-allelopathy on AOS of Casuarina equisetifolia Forst seedling was studied using a nutritional cultivation method.The water extract of the roots from 15-year C.equisetifolia Forst was used for the self-allelopathy.The results indicated that the AOS lost its original balance,and that the AOS greatly accumulated in C.equisetifolia Forst under self-allelopathy.The permeability of plasma membrane increased,while the membrane structure and function were damaged;the MDA content and the membrane lipid peroxidation increased;the O2- generation rate and H2O2 in leaf cells increased;the AOS became imbalanced;AOS accumulated greatly in leaf cells;the AOS defense system was damaged;the activities of SOD,CAT,GR and POD decreased;and,the ability of cells in clearing O2- and H2O2 was diminishing.
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