摘要:
建立了一种适用于食品中对羟基苯甲酸酯类(甲酯、乙酯、丙酯、丁酯)等常见防腐剂测定的简便快速的前处理方法.样品用环己烷/乙酸乙酯混合液(1:1,V/V)提取,通过加速溶剂萃取仪(ASE) 萃取,萃取液经凝胶渗透色谱仪(GPC)净化后,进GC-FID检测.4种对羟基苯甲酸酯在0.5～40.0 mgkg-1范围内呈良好的线性关系,对于.0 g的样品检出限分别为0.5mgkg-1、0.5mgkg-1、0.6 mgkg-1、0.6 mgkg-1 (S/N≥3),添加平均回收率8%～110%,RSD<10%.与国标前处理方法对照,本法具有灵敏度高、选择性好、回收率高、可靠性强、简单快捷等优点.
Abstract:
A rapid and convenient pre-treatment method for the detection of p-hydroxy benzoate,a category of commonly used food preservatives including methyl ester,ethyl ester,propyl ester and butyl ester,was developed.Samples were extracted using an accelerating solvent extraction device(ASE) with a cyclohexane-acetoacetate mixture(1:1,v/v) as the solvent.The extracted mixtures were then purified by chromatograph of gel permeation(GPC) and assayed by using the detector GC-FID.A satisfactory linear relationship was found in the range of 0.5-40.0 mgkg-1 for 4 parabens.The sensitivity on 5.0g sample was 0.5 mgkg-1,0.5 mgkg-1,0.6 mgkg-1 and 0.6 mgkg-1,(S/N≥3).The average recovery rates ranged between 85%-110%,and RSD was less than 10%.Compared to the currently available method,this newly developed method had a high sensitivity,selectivity,recovery rate and reliability.It was also simple and quick to operate and suitable for analytical applications.
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