摘要:
以24份杂交稻品种和18份杂交稻亲本为材料,利用SSR分子标记进行DNA指纹图谱构建和遗传多样性分析.24对引物在研究材料中共检测到74个多态性片段,平均每对引物的多态性片段为3.1个.基于24个SSR标记的扩增结果,利用NTSYS 2.10e软件计算Dice遗传相似系数,并建立UPGMA聚类图,结果表明: 42份材料之间的相似系数变化范围为0.0～1.00,在遗传相似系数0.8处可以将42份材料分为个类群,较好地反映了供试材料的亲缘关系,可为水稻育种中亲本选择提供参考.同时建立了42份材料×12个SSR标记的指纹图谱,为杂交水稻品种纯度和真伪鉴定提供科学数据.
关键词:
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水稻 /
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SSR /
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指纹图谱 /
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遗传多样性
Abstract:
SSR was used to construct the DNA fingerprints and analyze the genetic diversity of 24 hybrid rice cultivars and 18 parental cultivars.Twenty-four SSR primers amplified a total of 74 polymorphic DNA bands,averaging 3.1 bands per primer.Based on the amplification of 24 markers,genetic similarity coefficient of dice was calculated using the software,NTSYS 2.10e,and a dendrogram of genetic relationship was constructed using UPGMA method.The results showed that the genetic similarity among 42 cultivars ranged from 0.60-1.00,that 42 cultivars were classified into six cluster groups with the similarity coefficient of 0.68,and that the genetic relatives among cultivars was well revealed.It would provide a scientific basis for selection of parents for cross breeding rice.A fingerprint of SSR for 42 cultivars at the 12 primer loci was obtained,which would provide scientific data for identification as well as determination of purity of the hybrid rice.
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