Colonization and succession of two probiotic microorganisms in eel
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摘要: 对鳗鲡分别人工灌喂含有益生菌A0209CDC(枯草芽孢杆菌,Bacillus subtilis)和A31009NA(少动鞘氨醇单胞菌,Sphingomonas paucimobilis)的饲料,于停止灌喂后的第1、2、3、4、5、7、9、15、20、30 d进行细菌的分离培养,研究试验菌株A31009NA和A0209CDC在鳗鲡消化道内的定植与演替规律.结果显示:A0209CDC能在鳗鲡胃内定植至少7 d,在肠道内定植至少17 d;A31009NA能在鳗鲡胃内定植至少 d,在肠道内定植至少9 d;消化道细菌总数与益生菌数量同步变化.试验结果表明:试验用益生菌菌株A0209CDC和A31009NA均能在鳗鲡消化道内定植,并具有从胃至肠的迁移规律;人为添加具定植能力的益生菌时,将对养殖动物消化道菌群数量产生显著影响.Abstract: Two probiotic bacteria,A40209CDC4(Bacillus subtilis)and A31009NA(Sphingomonas paucimobilis),separated from the microflora in the intestinal duct of cultured European eel(Anguilla anguilla)were used to study the microbial colonization and succession.After being fed once per day the diet containing the two microorganisms for 5 days,the tested eels were asceptically dissected for bacteria isolation from their digestive canal at the 1st,2nd,3rd,4th,5th,7th,9th,15th,20th and 30th days.The result showed that(a) A40209CDC4 could colonize in the stomach for at least 7 days and in the intestines for at least 17 days;(b) A31009NA could colonize in the stomach for at least 4 days and in the intestines for at least 9 days;and(c) the changes of the total bacterial counts in the digestive tracts and those of the A40209CDC4 or A31009NA were parallel.The result indicated that A40209CDC4 and A31009NA could colonize well in the digestive canal of the eel,and could transfer from the stomach to the intestine;the microflora in the intestinal duct of the cultured fish could be considerably affected by feeding the fish with the probiotic bacteria.
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Key words:
- probiotic microorganisms /
- eel /
- digestive canal /
- colonization /
- succession
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[1] OLSSON C,WESTERDAHL A,CONWAY P L,et al.Intestinal colonization potential of turbot (Scophthalmus maximus L.) and dab (Limanda limanda)-associated bacteria with inhibition effects against Vibrio anguillarum[J].Appl Environ Micro,1992,58(3):551-556.
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