Green fluorescent protein gene transformation on Fusarium oxysporum f. sp. niveum strain, FOV-135
-
摘要: 建立并优化了PEG-CaCl2介导的原生质体转化体系,成功地将绿色荧光蛋白基因转入到西瓜尖孢镰刀菌FOV-135菌株中。转化子连续转接5代能够稳定遗传,荧光强度良好,每微克质粒DNA可获得4~6个性状稳定的转化子。PCR验证也表明gfp基因已转入到菌株FOV-135中。
-
关键词:
- 尖孢镰刀菌西瓜专化型 /
- 绿色荧光蛋白 /
- 原生质体转化
Abstract: The gene coding for green fluorescent protein(GFP) was successfully transferred to Fusarium oxysporum f.sp.niveum strain,FOV-135,by using the protoplast-mediated genetic transformation system.The transformants were stable after five successive subcultures.Four to six stable transformants could be obtained from one microgram of plasmid DNA.The PCR amplification confirmed that the gfp gene had been transformed to FOV-135. -
[1] NAHALKOVA J,FATEHI J.Red fluorescent protein (DsRed2) as a novel reporter in Fusarium oxysporum f.sp.lycopersici[J].FEMS Microbiology Letters,2003,225:305-309. [2] NONOMURA T,TAJIMA H,KITAGAWA Y,et al.Distinguishable staining with neutral red for GFP-marked and GFP-nonmarked Fusarium oxysporum strains simultaneously colonizing root surfaces[J].Journal of General Plant Pathology,2003,69:1,45-48.
点击查看大图
计量
- 文章访问数: 3122
- HTML全文浏览量: 151
- PDF下载量: 2378
- 被引次数: 0