摘要:
研究日本鳗鲡(Anguilla Japonica)消化道组织中的肥大细胞组织化学性质,观察肥大细胞胞浆颗粒的异质性。采用改良甲苯胺蓝(MTB)、阿利新蓝-沙黄O(AB/SO)、甲基绿-派洛宁(MG-P)、和天青Ⅱ-伊红-瑞氏混合液等4种组化染色法。用阿利新蓝-沙黄O染色法显示日本鳗鲡消化道的肥大细胞效果较好;甲基绿-派洛宁(MG-P)、和天青Ⅱ-伊红-瑞氏混合液染色效果一般,被染的肥大细胞数量很少;改良甲苯胺蓝甚至观察不到肥大细胞。日本鳗鲡肥大细胞数量不多,主要分布于食管、胃和肠道等部位黏膜层的黏膜上皮下方、固有层结缔组织,少量分布在黏膜下层结缔组织,并且有沿血管周围分布的特点;肥大细胞体积小,且多呈圆形、椭圆形、梭形及不规则形。AB/SO染色法效果最好,适用于日本鳗鲡肥大细胞的鉴定;改良甲苯胺蓝染色法不适合于日本鳗鲡肥大细胞的鉴定。
Abstract:
The purpose of this experiment was to investigate the histochemical property of the mast cells in the digestive tract tissue of Anguilla Japonica and observe the heterogeneity of cytoplasm granules of mast cells.This experiment used improved toluidine blue staining(MTB),Alcian blue Safranin O staining(AB/SO),methyl green-Pyronine staining(MG-P) and AzureⅡ-Eosin-Wright mixture staining.The mast cells in the digestive tract of A.Japonica were displayed better by Alcian blue Safranin O staining.The effect of methyl green-Pyronine staining (MG-P) and AzureⅡ-Eosin-Wright mixture staining was generic,and few mast cells were dyed.Even the improved toluidine blue staining couldn’t show the mast cells.Small amount of mast cells mostly distributed in connective tissues of lamina propria and under mucosa epithelium in mucous layer of esophagus,stomach and intestine. While less lied in connective tissues of submucosa,and intended to be around blood vessels.The shapes of the mast cells which were small in size were circular,ellipse,spindle and irregular.The effect of Alcian blue Safranin O staining was the best,which was applicable to identification of the mast cell of A.Japonica.The improved toluidine blue staining was not applicable.
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