应用巢式PCR检测鸭圆环病毒浙江株及其全基因序列分析

万春和; 施少华; 程龙飞; 傅光华; 陈红梅; 黄瑜

应用巢式PCR检测鸭圆环病毒浙江株及其全基因序列分析

1.
福建省农业科学院畜牧兽医研究所, 福建, 福州, 350013;
2.
福建省畜禽疫病防治工程技术研究中心, 福建, 福州, 350013

基金项目:

现代农业产业技术体系建设专项资金;福建省财政专项-福建省农科院科技创新团队建设基金(STIF-Y02);福建省农科院青年人才创新基金(2008 QB-6;B2007QJ20)

详细信息

通讯作者:
黄瑜(1965- ), 男,博士,研究员,从事动物传染病研究(E-mail:huangyu_815@163.com)

中图分类号: Q78;S85
计量
- 文章访问数: 2657
- HTML全文浏览量: 124
- PDF下载量: 337
- 被引次数: 0
出版历程
- 收稿日期: 2009-07-29
- 修回日期: 2009-09-17
- 刊出日期: 2009-10-15

Nested PCR detection and sequence analysis on Zhejiang duck circovirus

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

摘要

摘要: 应用巢式PCR(nested PCR)技术对收集自浙江温州种番鸭进行鸭圆环病毒核酸扩增,并对测定的序列进行分析,结果用套式引物扩增出目的条带片段大小约为340 nt。根据序列测定结果设计1对反向引物用于测定病毒基因组余下的基因片段。序列分析表明该圆环病毒基因组全长为1995 nt,其ORF-V1编码292个氨基酸,ORF-C1编码257个氨基酸。序列分析表明,本试验株与番鸭分离株(GenBank登录号EF451157)和半番鸭分离株(GenBank登录号EU344804)的同源性最高,均高达99.2%,与鸭圆环病毒欧洲(德国)分离株和北美(美国)分离株在同一支遗传进化关系上。
- 鸭圆环病毒 /
- 巢式PCR /
- 序列分析
Abstract: Using the nested PCR technology,the duck circovirus(DuCV) nucleotide fragment,which was isolated from Wenzhou,Zhejiang,was amplified and its sequence analyzed.The result showed that the obtained segment was about 340nt in length.Based on the determined sequence,two primers were applied for the construction of the remaining genomic sequence by inverse PCR.The genome of the Zhejiang DuCV had a length of 1995nt.Its ORFV1 encoded a replication protein of 292 amino acids,while the ORF-C1 encoded a capsid protein of 257 amino acids.The strain had highest sequence similarities(i.e.,99.2%) with EF451157 detected from the muscovy duck and EU344804 detected from the mule duck in Fujian.Phylogenetic analysis with Lasergene v 7.1 DNAStar software by Clustal W method showed that it had the same sub-lineage with the European strain detected in German mulard duck and the North American strain found in the American Perkin duck.
- duck circovirus /
- nested PCR /
- sequence analysis

HTML全文

参考文献(3)

[1]	YPELAAR I,BASSAMI M,WILCOX G,et al.A universal polymerase chain reaction for the detection of psittacine beak and feather disease virus[J].Veterinary Microbiology,1999,68(1-2):141-148.
[2]	TODD D,WESTON J,SOIKE D,et al.Genome sequence determinations and analyses of novel circoviruses from goose and pigeon[J].Virology,2001,286(2):354-62.
[3]	TODD D,WESTON J,BALL N,BORGHMANS B,et al.Nucleotide sequence-based identification of a novel circovirus of canaries[J].Avian Pathology,2001,30(4):321-325.