摘要:
从福建省福农生化有限公司排污口的土壤中筛选到1株对乐果有较好降解效果的菌株阴沟肠杆菌,为了使该菌能更好的应用于乐果残留污染的治理,采用正交试验法L16(44×23)研究各培养条件对该菌降解乐果的降解效能的影响,结果表明,该菌降解乐果的最佳发酵条件组合为:碳源为蔗糖、离子为CuCl2(10 mmolL-1)、乐果浓度100 mgL-1、培养时间36 h、培养温度30℃、pH值8、氮源(酵母3 gL-1),降解率达到58.90%;运用DPS数据处理系统分析各类因子对菌降解乐果的影响,得出离子对其影响最为显著,pH值、培养温度、乐果浓度、培养时间等也依次对菌降解乐果有较大的影响.
关键词:
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阴沟肠杆菌 /
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乐果 /
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降解 /
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优化 /
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正交试验
Abstract:
By using selective cultural media,Enterobacter closcae was isolated from the soil around a pesticide factory.For evaluating factors affecting E.closcae's potential in degrading residual dimethoate,a L16(44×23)orthogonal test was used for the design of the experiment.The results showed that sucrose as the conformable carbon source,10 mmolL-1 Cu2+,100 mgL-1 dimethoate,30 ℃,initial pH 8.0,3 gL-1 yeast extract as the conformable nitrogen source,and 36 h cultivation to yield a degrading rate of 58.90%.Statistical analysis by using DPS Data Processing System revealed that the ion exerted the most significant impact on the degradation rate,followed by pH,culture time,dimethoate concentration,temperature and carbon source in that order.
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