影响胡萝卜种子蛋白质提取的几种因素探讨

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基金项目:

厦门市科技项目(3502Z20062004)

Factors affecting protein extraction from carrot seeds

1.
Xiamen Agriculture Product Quality and Safety Testing Center, Xiamen, Fujian 361012, China;
2.
Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen, Fujian 361012, China

摘要: 探讨了提取液比例、pH值、提取缓冲液、浸提时间、PVP用量、提取介质、SDS等因素对胡萝卜种子蛋白质提取的影响.结果表明:蛋白质的提取受料液比、pH值、浸提时间、缓冲液种类、提取介质的影响较大.初步确定,在4℃条件下,胡萝卜种子蛋白提取的较佳条件为:料液比(W:V)1:10,pH值8.0的50mmolL-1Tris-HCL缓冲液,浸提时间1 h.
- 胡萝卜 /
- 种子蛋白 /
- 料液比 /
- 提取缓冲液 /
- 浸提时间 /
- 提取介质
Abstract: Factors including the ratio of sample to solution(W:V),pH value,buffers,time,amount of PVP,media and SDS that might affect the extraction of proteins from carrot seeds were studied.The results showed that the extraction was significantly affected by the ratio of sample to solution,pH,buffers,time and medium composition.Based on the preliminary results,protein extraction from carrot seeds for 1.0 h at 4℃ in 50 mmolL-1 Tris-HCl buffer with a 1:10 ratio of sample to solution and pH 8.0 produced the best result.
- carrot /
- seeds protein /
- ratio of sample to solution /
- extraction buffer /
- extraction time /
- extraction mediums

[1]	庄东红,黄秀丽,胡忠,等.影响南瓜种子蛋白溶出率几种因素的初步探讨[J].汕头大学学报,2004,19(4):28-32.
[2]	颜启传,苏菊萍,张春荣.国际农作物品种鉴定技术[M].北京:中国农业科学技术出版社,2004:98.

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