摘要:
用RAPD分子标记技术对18个黑麦草(Loluim sp)品种进行遗传多样性研究.从110个10 bp的随机引物中筛选获得26个多态性引物,然后对18份黑麦草种质资源的基因组DNA进行扩增,共获得197条DNA扩增片断带.其中多态性带187条,平均多态检出率为94.9%.18份材料的Jaccard相异系数为0～0.8467,采用UPGMA法聚类分析,结果显示:雅晴、特高、沃土、潘多拉、安格斯、意大利黑麦草、邦德、普通一年生、嘉宝、绿神、普通二倍体、小黑麦为一群,匹克、凯琳特、顶峰、明星、首相为一群,冬牧70为单独一群.
Abstract:
RAPD molecular-marker technique was used to detect the genetic diversity among 18 ryegrass germplasms.Their genomic DNA were tested with RAPD using 26 10-mer primers screened from 110 arbitrary 10-mer primers.A total of 197 RAPD bands were obtained,in which 187 were polymorphic.The average percentage of polymorphic bands was 94.9%.The variation range of genetic distances was from 0 to 0.846 7.By a UPGMA cluster analysis on the RAPD molecular-markers,these ryegrass varieties were divided into three groups:(1) Yatsyn,Tetragold,Pandora,Abundant,Angus,Italian Ryegrass,Barwoltra,Normal Annual Ryegrass,Green Spirit,Normal Diploid and Triticale;(2) Peak,Cailente,Pinnacle,Primer and Bright Star;and(3) Dong Mu 70.
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