湿地松组培快繁优良基因型的筛选

Selection of slash pine elite genotype for tissue culture and fast propagation

摘要: 以胚苗顶芽为外植体,开展湿地松离体培养初代培养基、增殖培养基的优化及组培快繁优良基因型的筛选试验。结果表明:最佳初代培养基为B5+6-BA 2.0 mg•L-1;年增殖率最大的增殖培养基为B5+6-BA 2.0mg•L-1+IBA 0.1 mg•L-1;试验从湿地松普通家系中筛选出增殖能力强的优良基因型132个,筛选出抗病家系优良基因型2个。

Abstract: Using the sprouts of slash pine as explants,medium formulation for the first generation and multiplication was optimized.The results showed that the optimum medium for the first generation was B5+6-BA 2.0 mg•L-1,and B5+6-BA 2.0 mg•L-1 +IBA 0.1 mg•L-1 for multiplication.The elite genotypes selected for the purposes included 132 from the non-antidisease and 2 from the antidisease families of the slash pine.