Isolation,screening and identification of methamidoghos-degrading bacteria from habitats at a pesticide factory
-
摘要: 对福建省福农生化有限公司不同生境中的甲胺磷降解细菌进行分离,用磷脂脂肪酸分析方法对菌进行鉴定,同时对降解细菌的生境特征进行分析。结果表明:对甲胺磷有降解作用的细菌共有22个属、28个种、67株菌株;在沟中污泥中分离到的降解细菌有8个属、12个种、35株菌株,其种类和数量多于其他的生境;而在各类降解细菌中,芽孢杆菌属和假单胞菌属分别有21株和19株,占分离到细菌总量的59.7%,其中的蜡状芽孢杆菌(Bacillus cereus)和恶臭假单胞菌(Pseudomonas putida)在以上3种生镜中大量存在。Abstract: To study methamidoghos-degrading bacteria in different habitats at a pesticide factory,soil samples from sewage,drainage ducts and surroundings at Fu-Nong Biochemistry Co.,Ltd in Fujian were collected for the isolation.Sixty-seven bacterial stains were isolated employing media containing high concentration of methamidoghos followed by identification using the fatty-acid analysis.They were determined to belong to 22 genera.In the 3 different types of habitats,35 microcial stains belonging to 8 genera were found.The numbers of Bacillus and Pseudomonas strains were 21 and 19,respectively.In combination,they amounted to 59.7% of the total.Among them,Bacillus cereus and Pseudomonas putida could grow in soils of all the sampling sites.
-
Key words:
- methamidophos /
- degrading bacterium /
- different habitats /
- isolation /
- screening /
- identification
-
[1] STEGER K,JARVIS A,SMARS S,et al.Comparison of signature lipid methods to determine microbial community structure in compost[J].Journal of Microbiological Methods,2003,55(2):371-382. [2] PCRKOVICH B S,ANDERSON T A,Kruger E L,et al.Enhanced Mineralization of C14 Atrazine in Koehia Scoparia Rhizosphere Soil from a Pesticide Contaminated Soil[J].Pestic Sci,1996,46:391-396.
点击查看大图
计量
- 文章访问数: 2534
- HTML全文浏览量: 91
- PDF下载量: 739
- 被引次数: 0