摘要:
研究组织特异表达基因对揭示植物生长发育机制具有重要的意义。通过生物信息学、RT-PCR及荧光定量PCR方法鉴定了2个水稻特异表达基因,即Os01g18840叶特异和Os01g28500茎叶颖特异表达基因。通过检测它们在PEG、NaCl、ABA及暗处理下的表达情况,发现在各种胁迫处理下,Os01g18840基因的表达受到抑制,Os01g28500基因的表达受到诱导。进一步分析它们的基因及蛋白结构特点,发现Os01g18840蛋白含有1个卷曲螺旋结构域,是1个中间纤维蛋白;Os01g28500蛋白含有1个SCP类似结构域,是与病原反应相关的蛋白。另外,对它们上游启动子区的顺式元件进行分析,结果表明它们都含有大量的与叶片、叶肉细胞或绿色组织中表达相关的顺式作用元件。
关键词:
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器官特异表达 /
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水稻 /
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基因 /
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顺式元件
Abstract:
Research on tissue-specific expressed genes is essential for the understanding of the molecular mechanism of plant growth and development. By means of bioinformatics, RT-PCR and real-time PCR, two tissue-specific expressed genes in rice, Os01g18840 and Os01g28500, were identified. The Os01g18840 gene was specially expressed in the leaves, and Os01g28500 in the stems, leaves and glumes. Furthermore, under the stress treatments of PEG, NaCl, ABA or in the dark, the expression of the Os01g18840 gene was reduced, but that of the Os01g28500 gene significantly induced. Analyses on the structures of the genes and proteins revealed that the Os01g18840 gene encoded an intermediate filament protein containing one coiled-coil domain, while the Os01g28500 protein had a SCP-like domain relating to pathogenic infection. In addition, the analysis on their upstream promoter region indicated that there were a large number of cis-elements relating to leaf, mesophyll cell or green tissue expression.
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