白羽半番肉鸭坦布苏病毒的分离鉴定及结构蛋白基因分析

傅光华; 陈红梅; 黄瑜; 万春和; 傅秋玲; 程龙飞; 施少华; 林芳; 林建生

白羽半番肉鸭坦布苏病毒的分离鉴定及结构蛋白基因分析

1.
福建省农业科学院畜牧兽医研究所, 福建福州 350013

基金项目:

现代农业产业技术体系建设专项(CARS-43);福建省种业创新专项(2011FJZY-9);国家自然科学基金项目(31201936);福建省科研计划项目——省属公益类科研院所基本科研项目(2011R1025-8)

详细信息

通讯作者:
黄瑜(1966-),男,博士,研究员,主要从事动物传染病研究(E-mail:huangyu_815@163.com)

中图分类号: S852
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- 被引次数: 0
出版历程
- 收稿日期: 2012-09-07
- 修回日期: 2012-10-11
- 刊出日期: 2012-12-18

Identification and Sequence Analysis of Tembusu Virus Isolated from White-feather Mule Ducks

1.
Institute of Animal Husbandry and Veterinary Medicine of Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 自肝脏表现不规则出血的白羽半番肉鸭脏器中分离获得1株病毒(命名为ZZ40株),经(RT-)PCR检测为鸭坦布苏病毒,可被鸭坦布苏病毒高免阳性血清特异性中和。应用鸭坦布苏病毒特异性引物从该株病毒基因组中成功获得结构蛋白基因片段,该片段与近年来我国分离的禽源坦布苏病毒株核酸序列同源性均在99.1%以上,而与蚊媒源坦布苏病毒MM-1775株同源性仅为88.6%;分子进化分析表明该株病毒与BYD-1等近年来的鸭坦布苏病毒分离株共处于一个进化分支。以上结果表明,我国白羽半番肉鸭存在坦布苏病毒感染,且该分离毒株的抗原性与我国早期分离于种(蛋)鸭的坦布苏病毒差异不明显。
- 坦布苏病毒 /
- 白羽半番肉鸭 /
- 分离鉴定 /
- 结构蛋白
Abstract: A viral isolate (named as ZZ40) was abtained from liver of white-feather mule ducks with irregular hemorrhagic hepatitis, and were identified as duck tembus virus (DTMUV) by RT-PCR. The virus was specifically neutralized by anti-DTMUV hyperimmune serum. Gene fregments were abtained by using three sets of primers aimed at viral structural genes, and shared no less than 99.1% nucleotide sequence identity with those of avian tembus viruses isolated recently, and no more than 88.6% with that of Mosquito-borne tembus virus MM-1775 strain. Phylogenetic analysis showed that ZZ40 strain clustal into the same clade with those duck viruses isolated recently. These results indicated that the white-feather mule ducks in this case was infected with duck tembusu virus, which has no abviously variation in antigenicity compared with those of the early isolates of duck tembusu virus from egg-laying duck.
- tembusu virus /
- white-feather mule duck /
- isolation and identification /
- structural protein

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参考文献(5)

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