Screening of Staphylococcus cohnii for Bioproduction of 5-aminolevulinic Acid
-
摘要: 以发酵产生5-氨基乙酰丙酸(ALA)为目标,从自然界进行菌株的随机筛选,结果得到4株可生产5-氨基乙酰丙酸的细菌LA-2、LA-4、LA-7、LA-10。摇瓶发酵LA-10产量最高,达6.88 mg·L-1。在常规生理生化特性测定基础上,结合16S rDNA序列分析,LA-10鉴定为科氏葡萄球菌(Staphylococcus cohnii),命名为科氏葡萄球菌LA-10。Abstract: Four strains named LA-2, LA-4, LA-7 and LA-10 were distinguished by random screening of strains for bioproduction of 5-aminolevulinic acid.After shaking flask fermentation, the highest yield was 6.88 mg稬-1 from LA-10. The strain was identified as Staphylococcus cohnii based on the morphological, physiological , biochemical characteristics, and 16S rDNA analysis. It was named Staphylococcus cohnii LA-10.
-
Key words:
- 5-aminolevulinic acid /
- Staphylococcus cohnii /
- screening /
- fermentation
-
[1] SASIKALA C, RAMANA C V, RAO P R. 5-aminolevulinic Acid: A Potential Herbicide Insecticide from Microorganisms [J]. Biotechnology progress, 1994, 10(5):451-459. [2] MOAN J, PENG Q. An outline of the hundred-year history of PDT [J]. Anticancer research, 2003, 23(5A):3591. [3] FU W, LIN J, CEN P. Enhancement of 5-aminolevulinate production with recombinant Escherichia coli using batch and fed-batch culture system [J]. Bioresource Technology, 2008, 99(11):4864-4870. [4] 张德咏, 刘勇, 成飞雪, 等. 一种高产5-氨基乙酰丙酸的工程菌. 中国专利:200610136870.7 ,2007-06-06.
点击查看大图
计量
- 文章访问数: 2320
- HTML全文浏览量: 110
- PDF下载量: 346
- 被引次数: 0