Functional Analysis of a Candidate Gene in Relation to Stress Resistance Induced with Magnaporthe grisea from Japonica Variety Yunyin
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摘要: 在对粳稻云引受稻瘟病菌诱导的基因芯片分析的基础上,克隆了其中一个受稻瘟病菌诱导后表达量显著变化的基因。序列比对结果显示,该基因与籼稻AF529237.2序列及普通小麦 X51608.1 序列分别具有99.0% 与88.0% 的同源性,与磷酸核酮糖激酶的活性相关,在糖代谢中起到重要的作用。构建该基因的系统进化树,结果表明在小麦、大麦、玉米等禾本科作物中也有其同源序列,并且同源性较高。推测该基因可能参与磷酸核酮糖和磷酸戊糖的代谢,执行磷酸核酮糖激酶的功能,并构建了该基因的过表达载体、干扰表达载体与瞬时表达载体。Abstract: Based on gene chip of japonica rice cv. Yunyin induced with Magnaporthe grisea, one of the candidate genes up-regulated remarkably by Sichuan43's isolation was cloned. Sequence and phylogenetic tree analyses suggest that the gene is closest relative to Oryza sativa L. AF529237.2 and Tritieum aestivum X51608.1 with 99.0% and 88.0% identity, and may relate to phosphoribulo kinase which is a critical enzyme in calvin cycle. Subsequently, the overexpression, RNAi expression and transient expression vector were constructed to obtain corresponsive transgenic lines, respectively. All these transgenic lines generated were used to assess the function of the gene in the stress response.
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Key words:
- Japonica rice Yunyin /
- Magnaporthe grisea /
- candidate gene /
- functional analysis
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[1] ZEIGLER R S,LEONG S A,TENG P S.Rice Blast Disease[M].CAB International and IRRI,Wallingford,United Kingdom,1994:16-26. [2] SILUD D,NOTTENGHEM J L,THARREAU D. Evidence of a gene-for-gene relationship in the Oryza sativa-Magnaporthe grisea pathosystem [J]. Phytopathology, 1992,82:557-580. [3] RUMPHO M E,POCHAREDDY S,WORFUL J M,et al. Molecular Characterization of the Calvin Cycle Enzyme Phosphoribulokinase in the Stramenopile Alga Vaucheria litorea and the Plastid Hosting Mollusc Elysia chlorotica[J]. Molecular Plant,2009,2(6):1384-1396.
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