超临界流体萃取紫芝挥发油成分的GC-MS分析

吴建国; 王宏雨; 吴岩斌; 陈体强

超临界流体萃取紫芝挥发油成分的GC-MS分析

1.
福建中医药大学中西结合研究院, 福建福州 350108;
2.
福建省农业科学院食用菌研究所, 福建福州 350013

基金项目:

福建省科技合作计划重点项目(2008I0010);福建省财政专项——福建省农业科学院科技创新团队建设项目(CXTD2011-24)

详细信息

通讯作者:
陈体强(1968-),男,副研究员,主要从事药用菌提取加工及天然产物研究(E-mail:chen_tiqiang@189.com)

中图分类号: TS224.4;Q934
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出版历程
- 收稿日期: 2012-02-17
- 修回日期: 2012-03-20
- 刊出日期: 2012-08-30

GC-MS Analysis of the Essential Oil from Ultrafine-Powder of Ganoderma sinense by Supercritical CO₂ Fluid Extraction

1.
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, China;
2.
Institute of Edible and Medicinal Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 为进一步研究紫芝挥发油的化学成分,采用超临界CO2流体萃取法从紫芝超细粉中提取分离挥发油,气相色谱-质谱联用分析其化学成分。从挥发油萃取物(1.47%,w/w)中检出46个峰(组分),鉴定了其中26个化合物(匹配度>83%),总相对含量占79.2%,主要成分有十六(烷)酸(18.88%)、(E)-十八碳-9-烯酸甲脂(18.74%)、(Z)-9-十八碳烯酸(24.33%)、(E)-2-癸烯醛(0.77%)、(E,E)-葵二烯醛(1.53%)、1,8,8-三甲基-2-(3-氧代环戊基)-三环[3.2.1.0(2,4)]辛烷-3,3-二腈(4.41%)、9-甲基-Z-1-十四碳-1-醇乙脂(1.20%)、(Z)-9-十八碳烯酸-2-羟基-1-羟甲基乙脂(1.95%)和E-11-十六烯-1-醇(2.38%)等。超临界流体萃取分离的紫芝挥发油成分及其相对含量有别于乙醚回流-水蒸气蒸馏法提取得到的挥发油成分。
- 紫芝 /
- 挥发油 /
- 超临界流体萃取 /
- 气相色谱-质谱
Abstract: Objective:Further to analysis chemical components in the essential oil(EO) of Ganoderma sinense. Methods:EO was extracted using Supercritical CO2 fluid extraction(SFE-CO2) technique from the ultrafine-powder of Ganoderma sinense, and detected by Gas chromatography-mass spectrometry (GC-MS). Results: 46 compounds were checked out from the EO extract(with 1.47% of extract rate, w/w), and 26 compounds(matching degree >83%) were identified representing 79. 2% of the total EO, including n-Hexadecanoic acid, (E)-9-Octadecenoic acid methyl ester, (E)-9- Octadecenoic acid and (E,E)-2,4- Decadienal, 9-Methyl-Z-10-tetradecen-1-ol acetate, 1,8,8-trimethyl-2-(3-oxocyclo- pentenyl)-Tricyclo[3.2.1.0(2,4)]octane-3,3-dicarbonitrile, (Z)-9-Octadecenoic acid-2-hydroxy-1- (hydroxymethyl) ethyl ester and E-11-Hexadecen-1-ol and so on. Collusion: The results indicated that chemical components and their relative contents of the EO extracted by SFE-CO2 were different from that by steam distillation(SD) after ether refluxing.
- Ganoderma sinense /
- essential oil /
- supercritical CO2 fluid extraction /
- Gas chromatography- mass spectrometry

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参考文献(3)

[1]	张裕强,郭立玮,刘史佳,等. 不同方法提取荜茇挥发油的GC-MS分析[J]. 质谱学报,2008,29(4):231-236.
[2]	陈体强,吴锦忠. 超微粉碎后超临界CO2 萃取灵芝孢子挥发油组分的GC-MS分析[J]. 天然产物研究与开发,2006a,18(6):982-985.
[3]	CHEN T Q, ZHAO X Y, WU J Z, et al. Supercritical fluid CO2 extraction, simultaneous determination of components in ultrafine-Powder of Ganoderma sinense by HPLC-ESI-MS method[J]. Journal of the Taiwan Institute of Chemical Engineers, 2011, 42(3): 428-434.