摘要:
测定了从球孢白僵菌侵染的茶丽纹象甲虫尸上分离的8个分离株的生长速率、产孢量和孢子萌发率等生物学性状,以及对茶丽纹象甲成虫的杀虫活性,旨在筛选出感染该虫的高致病力菌株。结果表明:在萨氏培养基(SDAY)上,8个分离株从菌落形态上可分为菌丝型和孢子型两类。其中,菌丝型菌株XJBb3001和XJBb3007生长速率最快,但菌丝徒长,未见明显产孢现象;菌株XJBb3005产孢量最大,为2.47×107个·mL-1,且都与其他菌株产孢量存在极显著差异;各菌株在孢子萌发率上不存在显著差异。采用浸虫法用1.0×107个·mL-1的孢子溶液处理茶丽纹象甲成虫后,菌株XJBb3005表现出最强的杀虫活性,第7d校正死亡率为100%,LT50仅为3.46d,LT90为5.45d,僵虫率达93.75%。
Abstract:
In order to rescreen high virulence strains against Myllocerinus aurolineatus, the growth rate, sporulation, germination rate and insecticidal activity of eight Beauveria bassiana isolates, which were isloated from dead body of M. aurolineatus, were investigated. The results showed that the eight isolates could be seperated into two groups: hyphae type and spore type on the SDAY medium. The hyphae type strains of XJBb3001 and XJBb3007 grew faster than the others, but they are useless hyphae and no producing spores. The strain XJBb3005 had the largest number of sporulation, and there were very significant differences in sporulation number between strain XJBb3005 and other isolates. There is no difference among the spore germination rate of the strain XJBb3002, XJBb3003, XJBb3004, XJBb3005, XJBb3006, XJBb3008. Bioassay evaluation were carried out by dipping in spore suspension (1.0?107spores穖L-1), and the results showed that the isolate XJBb3005 had highest virulent, and corrected mortality of M. aurolineatus reached 100% at 7 d after treatment. The LT50 value was 3.46 d,LT90 value was 5.45 d, and infected rate was 93.75%, respectively.
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