Taizishen's Resistance to Leaf Spot Disease Induced by Salicylic Acid
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摘要: 为探讨水杨酸SA对太子参叶斑病的诱抗效果以及有关酶活性的变化与SA抗病作用的可能联系,在离体培养条件下,运用水杨酸0.5~15mmolL-1 6种不同浓度处理太子参组培苗,在1~7d内测定叶片相关酶活性的变化,诱导培养7d后,用太子参叶斑病孢子悬浮液对组培苗进行抗性鉴定。结果表明,SA 5mmolL-1诱导处理后能明显增强太子参叶片内POD、PPO酶活性,降低CAT酶活性,其中POD活性比对照增加170%,PPO活性比对照增加293%,CAT活性比对照降低43.3%。6种浓度均能不同程度地诱导太子参对叶斑病产生抗性,以5.0mmolL-1水杨酸的诱抗效果最好,达到59.6%,诱抗效果极显著高于其他处理浓度。Abstract: Salicylic acid(SA)was applied on Taizishen(Pseudostellaria heterophylla)to induce the plant's resistance to the leaf spot disease.Enzyme activities relating to the disease-resistance were investigated.In vitro-regenerated Taizishen plantlets were treated under aseptic conditions with 6 different concentrations of SA,0.5-15 mmol-1,to determine the optimum SA induction level.Activities of various enzymes in the leaves were determined within 1-7 days after SA treatment.The disease-resistance of the treated plantlets was observed with the application of a water suspension of the leaf spot spores,7 days after the treatment.The results showed that(a)the activity of the enzymes,such as peroxidase and polyphenol oxidase,in the leaves was enhanced by SA treatment;(b)the catalase activity was significantly inhibited after induction by SA at a concentration of 5 mmol-1;and(c)POD activity increased by 170%,PPO increased by 293%,and CAT decreased by 43.3%,as compared to the control.SA concentration in the range of 0.5-15 mmol-1 appeared to be capable of inducing the disease-resistance in Taizishen.The best result seemed to be at the concentration of 5 mmol-1 SA,as the average effective rate was 59.6%,which was significantly higher than those achieved by the other treatments.
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Key words:
- induced resistance /
- salicylic acid /
- Taizishen /
- enzyme activity
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[1] 刘亚光,马超,冯蕾. 几种化学诱抗剂对大豆细菌性斑点病诱导抗性的研究[J]. 中国油料作物学报, 2008, 30(1):116-118. [2] 萧浪涛,王三根. 植物生理学试验技术[M]. 北京:中国农业出版社, 2005: 103-104.
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