福建省烟粉虱自然种群遗传多样性的RAPD和ISSR分析

郑宇; 赵建伟; 何玉仙; 黄建

福建省烟粉虱自然种群遗传多样性的RAPD和ISSR分析

郑宇^1,2,
赵建伟¹,
何玉仙¹,
黄建^2, ,

1.
福建省农业科学院植物保护研究所, 福建福州 350013;
2.
福建农林大学植保学院, 福建福州 350003

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项(2010R1026-1);福建省自然科学基金项目(2011J01117);福建省财政专项——福建省农业科学院科技创新团队建设基金项目(STIF-Y07)

详细信息

通讯作者:
黄建(1951- ),教授,博士生导师,研究方向:植物保护、害虫综合防治(E-mail:jhuang@fjau.edu.cn)

中图分类号: Q769
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出版历程
- 收稿日期: 2012-03-05
- 修回日期: 2012-04-24
- 刊出日期: 2012-05-30

Genetic Diversity of Bemisia tabaci(Gennadius) Natural Populations in Fujian Province Based on RAPD and ISSR Marker

1.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350003, China

摘要

摘要: 采用RAPD和ISSR2种分子标记分别对福建省7个地区8个烟粉虱自然种群的80份样品进行遗传多样性分析。筛选出4条RAPD引物和4条ISSR引物,从80份样品的DNA中分别扩增2240和1776条带,其中多态性条带分别为2000和1728条,说明福建地区烟粉虱自然种群遗传多样性较为丰富;聚类分析表明,福建地区8个自然烟粉虱种群可分为2大类群,采自龙海变叶木种群单独聚为1类,说明与其他种群存在较远亲缘关系,推测为不同生物型;遗传距离分析说明福建不同种群在遗传相似性上与地理分布有一定的相关性。
- 烟粉虱 /
- RAPD /
- ISSR /
- 遗传多样性
Abstract: Using RAPD and ISSR molecular marker technology,genetic diversity of 80 samples of 8 natural populations of Bemisia tabaci collected from 7 regions of Fujian province, China was studied in this paper. The 2240 and 1776 bands were amplified respectively with 4 RAPD primers and 4 ISSR primers from the DNA of 80 samples, and among them, 2000 and 1728 bands were polymorphic respectively. The results showed that genetic diversity of B. tabaci in natural populations were quite abundant in Fujian. According to the UPGMA clustering based on the genetic distance between the populations, the 8 populations were classified into 2 groups. One group was only Longhai population(host:Codiaeum variegatum), which could be illustrated that it kept at a distance with other populations on genetic relationship. We inferred that it was possible a different biotype with others. In addition, a certain correlation was observed between the geographic distance and the genetic distance of the populations.
- Bemisia tabaci(Gennadius) /
- RARD /
- ISSR /
- genetic diversity