鸭副粘病毒弱毒株的选育

基金项目:

福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y02);福建省农业科学院科技计划项目(B2010-02)

Selection of Attenuated Vaccine Strain of Duck Paramyxovirus

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

摘要: 应用SPF鸡胚成纤维细胞(CEF)连续传代培育出鸭副粘病毒弱毒疫苗株,并测定该弱毒株的部分生物学特性。结果显示:该弱毒株已失去对无母源抗体番鸭的致病性,在雏番鸭体内连续肓传5代,未见毒力返强现象;对CEF的TCID50稳定在10-6.0~10-6.5·(0.1mL)-1;无母源抗体的番鸭免疫不同代次弱毒后7d攻击鸭副粘病毒强毒,保护率均在90%以上。结果表明该弱毒株安全性好、遗传性稳定、免疫原性强,可用于制备鸭副粘病毒病疫苗。
- 鸭副粘病毒 /
- 弱毒株 /
- 选育
Abstract: An attenuated vaccine strain of Duck Paramyxovirus(DPMVa) was selected and obtained by passage serial within SPF chicken embryo fibroblast(CEF), and some biological property of it were detemined. The DPMVa was non-pathogenic to Muscovy ducks without maternal antibody; and was non-viulence through five serial passages in one-day-old Muscovy ducks; TCID50 to CEF stable between 10-6.0~10-6.5·(0.1 mL)-1. The protection rate of the attenuated strain was above 90% when challenged with a virulent strain of DPMV 7 days after vaccination. The results indicated that attenuated vaccine strain had excellent safety, effective immunogenicity and genetic stability, which could be used as a vaccine against DPMV.
- duck paramyxovirus /
- attenuated vaccine strain /
- selection

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