Selection of Attenuated Vaccine Strain of Duck Paramyxovirus
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摘要: 应用SPF鸡胚成纤维细胞(CEF)连续传代培育出鸭副粘病毒弱毒疫苗株,并测定该弱毒株的部分生物学特性。结果显示:该弱毒株已失去对无母源抗体番鸭的致病性,在雏番鸭体内连续肓传5代,未见毒力返强现象;对CEF的TCID50稳定在10-6.0~10-6.5·(0.1mL)-1;无母源抗体的番鸭免疫不同代次弱毒后7d攻击鸭副粘病毒强毒,保护率均在90%以上。结果表明该弱毒株安全性好、遗传性稳定、免疫原性强,可用于制备鸭副粘病毒病疫苗。Abstract: An attenuated vaccine strain of Duck Paramyxovirus(DPMVa) was selected and obtained by passage serial within SPF chicken embryo fibroblast(CEF), and some biological property of it were detemined. The DPMVa was non-pathogenic to Muscovy ducks without maternal antibody; and was non-viulence through five serial passages in one-day-old Muscovy ducks; TCID50 to CEF stable between 10-6.0~10-6.5·(0.1 mL)-1. The protection rate of the attenuated strain was above 90% when challenged with a virulent strain of DPMV 7 days after vaccination. The results indicated that attenuated vaccine strain had excellent safety, effective immunogenicity and genetic stability, which could be used as a vaccine against DPMV.
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Key words:
- duck paramyxovirus /
- attenuated vaccine strain /
- selection
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[1] 殷震,刘景华.动物病毒学:第2版[M].北京:科学出版社,1997:223-224. [2] NEROME K,SHIBATA M,KOBAYASHI S,et a1.Immunological and genomic analyses of two serotypes of avian paramyxovirus isolated from wild duck in Japan [J].J Virol,1984,50(2):649-653. [3] 张存,叶伟成,刘蔓雯,等.鹅与番鸭溃疡性胃肠炎病毒的分离鉴定[J].畜牧与兽医,2002,34(2):27-28.
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