摘要:
为研究亚硒酸钠添加水平为3 μg·mL-1时,秀珍菇液体培养菌丝体动态变化规律,采用3×10 L的全自动控制发酵罐液体培养秀珍菇富硒菌丝。选择发酵罐培养的生物生长量、培养环境、发酵液营养水平等动态因素,以菌球直径、菌球密度、液体培养基pH值、溶氧量、可溶性糖、可溶性蛋白等为测定指标,确定秀珍菇富硒液体培养菌丝体动态变化规律。结果显示:富硒组与对照组生长趋势基本一致,生物量、菌球直径、菌球密度三者随培养时间呈增长趋势,且变化显著相关。72 h培养结束时,对照组与富硒组生物量分别为1.92、1.81 mg·mL-1,菌丝硒含量分别为4.99、45.37 mg·kg-1,富硒培养生物有机硒转化率为53.37%。随培养时间的变化,对照组与富硒组培养液中可溶性糖与可溶性蛋白含量的动态变化趋势相对一致,显示随着菌丝体的生长不断被消耗,呈现不断下降趋势。
关键词:
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秀珍菇 /
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液体培养 /
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变化规律 /
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硒
Abstract:
Development of Pleurotus geesteranus Singer mycelia in the submerged medium containing 3 μg added selenium (Se) per mL was studied in a 3×10 L auto-control fermentor. Biological characteristics, culture environment and medium nutrition including the diameter and density of the mycelial spheres, as well as the pH, DO, total soluble sugar and protein of the medium were monitored to determine the effect of the selenium addition. The results showed that (a) the mycelial growth was basically the same with or without the Se addition, (b) the biomass as well as the sphere’s diameter and density increased with incubation time, and (c) a significant correlation existed among the factors. At the end of 72h culture, the biomass in CK and Se groups were 1.92 and 1.81 mg·mL-1, respectively; the Se content of mycelium in the treatment group was 45.37 mg·kg-1 with an Se bioconversion efficiency of 53.37%. During incubation, the total soluble sugar in the culture medium slowly increased initially followed by a decline, but the total soluble protein remained constant on a decreasing trend.
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