摘要:
对低温胁迫下不同品种青花菜生长情况及SOD和POD酶活性进行分析,并以‘翠绿’品种为试材,研究外源ABA对植株低温胁迫的缓解作用。结果表明:(1)低温胁迫抑制株高、茎粗的增长,SOD、POD活性及可溶性糖含量显著降低;不同品种对低温胁迫的响应不同,‘翠绿’、‘蔓陀绿’品种受低温胁迫的影响较大,‘优秀’、‘DICICCO’(AU)品种受低温胁迫的影响较小。(2)100 mg·L-1 脱落酸(ABA)处理对提高‘翠绿’的株高、茎粗效果显著;50、100、150 mg·L-1 ABA处理的植株SOD、POD活性均显著高于对照(0 mg·L-1 ABA),其中,100 mg·L-1 ABA对SOD、POD活性的提高效果最为显著。(3)外源ABA可显著提高‘翠绿’植株可溶性糖含量,在低温处理15 d并恢复处理15 d后,50、100、150 mg·L-1 ABA处理的可溶性糖含量均显著高于对照并保持在较高水平。
Abstract:
The growth situation and activity variation of SOD and POD of different broccoli cultivars under cold stress were evaluated. After that, the relaxation effect of exogenous ABA on plant growth was studied by using broccoli cultivar ‘Cuilv’ (CL) as test material. The results showed that: (1) Exposure of broccolis to cold stress resulted in inhibition on plant height and stem diameter. Meanwhile, the activities of SOD and POD and soluble sugar content of the broccolis decreased significantly. However, different cultivars showed different responses to cold stress. The cultivar CL and MTL were greatly suppressed by cold stress, while cultivar YX and AU showed less influence. (2) ABA at 100 mg·L-1 improved plant height and stem diameter of CL significantly. The SOD and POD activities of plants treated by 50, 100 and 150 mg·L-1 of ABA were significantly higher than that of control. In addition, 100 mg·L-1 ABA increased mostly the activities of SOD and POD among all treatments. (3) Exogenous ABA significantly enhanced the soluble sugar content of CL. After 15 days of cold treatment followed by 15 days of recovery treatment, the soluble sugar contents of CL plants treated by 50, 100 and 150 mg·L-1 ABA were significantly higher than that of control and maintained at a high level.
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