摘要:
以高浓度的赖氨酸、苏氨酸或色氨酸作为培养基添加物,对水稻体细胞和性细胞无性系进行离体筛选。获得一批耐高浓度氨基酸的抗性愈伤系并再生成绿色植株。据T1代糙米氨基酸含量分析,9个突变体的17种氨基酸总量比原品种增加10.6～46.9%,7种必需氨基酸含量提高10.4～58.2%,其中赖氨酸提高7.8～42.3%。经主要农艺性状的变异系数分析,可育突变体在T1～T2代性状就相对稳定。本文讨论了抗性突变体离体筛选在水稻优质育种中的应用潜力。
Abstract:
The seed-derived calli and microspore-derived calli of rice were cultured on the medium supplemented with high concentration oi lysine, thrrouino or tryptophane for screening in vitro. Some resistants to high concentration of amino acid calli were isolated and then regenerated into green plants. By analysing the amino acid contents of brown rice in T1 generation, the total amount of 17 kinds of amino acid in 9 mutants was increased by 10.6-46.9%, the amount of 1ys, Thr, Met, Phe, Leu, Ileu and Val plus Cys was increased by 10.4-58.2%, among them, the contents of lysine was increased by 7.8-42.3%. With counting the C. V. of main agronomic traits, the fertile mutants in T1-T2 were stable. The possible potential of screening resistant mutants in vitro used for rice quality breeding was discussed.
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