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禽白血病毒单克隆抗体的特性

程由铨 Lucy F. lee Robert F. Silva Fugene J. Smith Lyman B. Crittenden

程由铨, Lucy F. lee, Robert F. Silva, Fugene J. Smith, Lyman B. Crittenden. 禽白血病毒单克隆抗体的特性[J]. 福建农业学报, 1986, 1(1): 1-10.
引用本文: 程由铨, Lucy F. lee, Robert F. Silva, Fugene J. Smith, Lyman B. Crittenden. 禽白血病毒单克隆抗体的特性[J]. 福建农业学报, 1986, 1(1): 1-10.
Cheng Youquan, Lucy F. lee, Robert F. Silva, Fugene J. Smith, Lyman B. Crittenden. CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO AVIAN LEUKOSIS VIRUSES[J]. Fujian Journal of Agricultural Sciences, 1986, 1(1): 1-10.
Citation: Cheng Youquan, Lucy F. lee, Robert F. Silva, Fugene J. Smith, Lyman B. Crittenden. CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO AVIAN LEUKOSIS VIRUSES[J]. Fujian Journal of Agricultural Sciences, 1986, 1(1): 1-10.

禽白血病毒单克隆抗体的特性

CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO AVIAN LEUKOSIS VIRUSES

  • 摘要: 建立了分泌抗禽白血病毒(ALV)结构蛋白P27和P19的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL22(属于IgG1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔35S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P19蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P19蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P19(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb-6AL42可与P27和pr76(群特异性抗原的前体蛋白)发生免疫沉淀。试验用McAb-6AL42作为第一抗体和兔抗P27血清的过氧化物酶标记物作为第二抗体(指示抗体)建立了双抗体夹心ELISA试验。用这种ELISA试验可区分未经稀释的卵蛋白中RAV-O和RPL-40群特异性抗原。
  • [7] Crittenden, L, B.,xue E, J.Smith, 1984, A comparison of test materials for differentiaLiag avian leak osis virus group-specific antigens of exogenous and endogenous origin, Avian Dis, 28:1055
    [8] De Boer, G. F. and A. D. E. Osterhaus. 1985, Application of moaoclonal antibodies in the avian leukosis virus gs antigen EL1SA。A vian Pathol. 14:3-55
    [13] lgnjatovic, J., and T. J. Bagust. 1983. Practical application of ELISA for detection of vertical transmission of leukosis virus in commercial layer hens, Avian pathol, 12:515-519
    [17] Parsons, S. J.,L. D. Wilson, C. M. Ely, J. T. Parsons, and D. C. Benjamin 1984, Monoclonal antibodies specific for the virion polypeptide, p27. of avian retrov:ruses, Hybridoma 3:25-31
    [18] purchase, H. G.,and L. N. Payne, 1984, Leuhosis/sarcuma group, in:Diseases of Poultry, 8th Ed,(M. S. Hofstad. H. J. Barnes. B. W. Calnelt, W, M, Reid, and H. W. Yoder, eds.), pp. 360-405.Iowa State University Press, Ames, Iowa
    [20] Robinson, H. L., and R. N. Eisenman, 1934. New findings on the congenita; transmission of avian leukosis virus, Science 325:417-419
    [21] Silva, R. F., and L. F. Lee, 1934, Monoclonal antibody-mediated immunoprecipitation of proteins from cells infected with Marek’s disease virus or turkey herpesvirus, Virology, 136:307-320.
    [25] Wilson,M. B. and P.K. Nakane, 1978. Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In:Immunofluorescenee and Related Techniques (W. Knapp, K. Holubar, and G. Wicks, eds.)pp, 215-234. Elsevier/Nurth Holland Biomedical Press, Amsterriam, Holland
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  • 刊出日期:  1986-09-15

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