摘要:
采用小苍兰五个品种的花器作组织培养,均获得愈伤组织和试管苗。将活性炭不同浓度(0.0%、0.2%、0.5%、0.8%),加入含2,4-D0.1毫克/升+BA0.1毫克/升的MS培养基中,可使小苍兰愈伤组织的分化率从25%提高到70.6%以上。在试验范围内,随着活性炭浓度提高,所分化的苗叶色渐趋青绿,清秀;但苗长得过高,不便移植。用矮壮素加入上述培养基中可使苗矮壮。试验结果表明,以附加矮壮素5毫升/升的达到秆粗0.3厘米的试管苗,其矮化百分率最高,其次为3毫升/升的。试验结果还表明,花器诱导的再生植株可以有两种途径:先芽后根和先根后芽。在供试各品种中,以鲜黄增殖速度最快,大白、桔红次之,粉红、土蓝再次之。
Abstract:
The promotive effects of abscisic acid on bud formation of common freesia callus were studied. The results are summarized as follows:1.The floral envelope was cultured on MS medium supplemented with BA 1 mg/1 and NAA 0,1 mg/1, The frequency of culli induction and bud formation were a little. But the floral axis of the common freesia cultured on MS medium added with IBA, IBA greatly stimulated root formation in the concentration of 0.5-2 mg/1.2.When MS medium added with BA 0.1 mg/1 and 2,4-D 0.1 mg/1, the activated carbon played an important role, as to promote the culli induction and bud formation. The optimum concentration of the activated carbon was 0.8%.3.Abscisis acid is obviously beneficial to promote the bud strong in the concentration of 5 mg/1.
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