摘要:
采用高效液相色谱分离,紫外254nm波长检测,对草莓、甜瓜、菜豆、夏橙、荔枝、空心菜的抗坏血酸含量进行测定,与经典的2.6—二氯靛酚滴定法比较,结果较为接近。0.008M磷酸盐缓冲液(pH=5),含有0.002M四丁基氢氧化铵反离子剂作为流动相。使用3%柠檬酸为提取液,并与醋酸、偏磷酸作为提取液进行比较,效果更好。回收率90%以上,最低检测限0.05μg,保留时间小于5分钟。这一研究说明该方法测定果蔬维生素C含量,具有简单、快速、灵敏的优点。某些果蔬品种间抗坏血酸含量差异较大,因此在培育优良品种时,应重视提高抗坏血酸的含量。
Abstract:
Some strawberry, melon, bean, orange,litchi and water spinach were analyzed for ascorbic acid content by high-performance liquid chor-matography (HPLC) with a UV detector at 254nm. The results were com-pared with the dyetitration method using 2.6-dichlorophenol-indophenol and the values of ascorbic acid by both HPLC and titration were comparable. The mobile phase was made of 0.008 M phosphate buffer (pH-5.0) with 0.002M tetrabtuyl ammonium hydroxide. Extraction procedures using 3% citric acid were compared with those using acetic acid and me-taphosphoric acid.The recovery values were more than 90% and minimum detectable quantity was 0.05μg. The study showed that HPLC was a simple, rapid, sensitive method for the analysis of ascorbic acid content in fruits and vegetables. The highly variable of ascorbic acid between bean and litchi varieties was obtained. Authers suggest that the variety with high content of ascorbic acid must be used as parent plant in future breeding work.
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