在试管中茎尖嫁接脱除柑桔黄龙病病原的研究
STUDY ON THE ELIMINAITON OF CITRUS HUANGLUNGBIN PATHOGEN BY SHOOT-TIP GRAFTING IN VITRO
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摘要: 1983～1986年间,我们开展了利用茎尖微芽嫁接脱除柑桔黄龙病病原的研究。结果从嫁接的85株苗中,获得3株茎尖嫁接苗。对茎尖嫁接苗和原病株(对照)进行生物学鉴定和电子显微镜检查,示明以茎尖嫁接苗的多芽枝段接种的6株椪柑实生苗,均无表现病状;在电镜下在其叶脉的筛管细胞内均无发现病原体。反之,用对照病株的多芽枝段接种的9株椪柑实生苗,经过5～9月,全部表现典型的黄龙病病状;在电镜下在其叶脉的筛管细胞内则观察到很多黄龙病的特征性的病原体。这说明应用茎尖嫁接法可成功地脱除母树携带的黄龙病病原,获得无病的柑桔植株。Abstract: A study on the elimfnation of citrus Huanglungbin pathogen by shoot tip grafting (STG) in vitro was made from 1983 to 1986. The result showed that 3 budlings by STG were obtained from 85 Huanglungbin takan seedlings. The STG budlings and original diseased Pankan seedlings (control) were indexed for the presence of Huanglungbin pathogen respectively by the methods of biological test and electron microscope examination. The indicated Ponkan seedlings inoculated with the buds from STG budlings did not show any Huanglungbin symptoms, and in the sieve cells of leaf vein of indicated seedlings no Huanglungbin pathogen was discovered under electron microscops. On the other hand, the typical symptoms appeared on the indicated Ponkan seedlings inoculated with the buds of control plant after 5-9 months and many typical pathogens (rickettsia-like or bacteria-like organisms) were found in their sieve cells of leaf vein under electron microscope. These results showed that the Huanglungbin pathogen could be eliminated by STG.
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[3] 柯穗等,1986,通过苑丝子成功地将黄龙病病原从柑桔传到长春花。福建省农科院学报1(1):11~160 [7] 赵学源等,1981,柑桔黄龙病对青霉素和四环素的反应。中国柑桔4:17. [8] 赵学源等,1983,利用茎尖嫁接脱毒培育无裂皮病的脐血橙。植物保护学报10(3):166. [11] Navarro, L, 1981.Citrus shoot-tip grafting in vitro(STG)and its application, A review,Proc, Int soc.Citriculture.452~456. [16] Nauer,E.M,,C,N,Roistacher,,T;L,Carson and T,nLurashige,1983,In vitro shoot-tip grafting to Eliminate citrus,viruses and virus-like pathogens produces uniform budlines, H ortscience, 18 (3):308~309. -
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