摘要:
以籼型杂交水稻F1为材料,研究化学诱导水稻孤雌生殖子房的离体培养。在SK3和N6附加GA30.5～2ppm培养基上培养7～15天,获得四优30等5个组合的孤生子房植株。其中以5～10天日龄及SK3和N6培养基的成苗率较高。在0.5～2ppm范围内,GA3能诱导孤生子房直接成苗;NAA和2,4—D可诱导形成愈伤组织;而KT和TRIA则未能诱导直接成苗或形成愈伤组织。多数孤生植株由幼胚部位直接长成,少数孤生子房先在幼胚部位形成愈伤组织,转移到分化培养基上再分化出绿色植株。子房植株有单倍体和自然加倍二倍体两种类型。孤生子房植株的主要农艺性状在PO2～PO3代是相对整齐一致的。
Abstract:
The parthenogenetic ovaries induced by chemicals (PCPA) in vitro were hybride rice (indica) Fl’s.when they were cultured for 7-15 on SK3 and N6 media supplemented with GA3 0.5-2 ppm,the parthenogenetic plantlets of five combinations,such as Siyou No.30, were obtained.The regeneration rate of 5-10-day-old parthenogenetic ovaries grown on SK, and N6 was higher than that on MS. At a range of 0,5-2ppm, GA3 was able to induce parthenogenetic ovaries to form plantlets directly; NAA and 2, 4-D, to form calli.But KT and TRIA were not able to,Most of the parthenogenetic plantlets developed directly out of embryos: a few parthenogenetic ovaries first formed calli and then regenerated into green plantlets after their transfer on MS. Some of the parthenogenetic plantlets were haploids,others were natural diploids. Parthenogenetic plants showed their relative regu-lartiy and consistence in main argronomic traits during the PO2-PO3 generations.
点击查看大图
下载:
