萍藻重组研究——Ⅰ.无藻萍的获得及其验证技术研究
THE RECOMBINATION OF ANABAENA——FREE AZOLLA WITH ANABAENA AZOLLAE I. THE ACQUIREMENT AND IDENTIFICATION OF ANABAENA——FREE AZOLLA
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摘要: 应用红萍茎尖培养和切除雌雄混养的大孢子果顶端囊群盖和漏斗状膜,可以去除鱼腥藻,成功地得到无藻红萍(简称无藻萍)。经石蜡切片、微型玻管从叶片共生腔中吸取粘液作显微观察、无氮培养、固氮酶活性测定、扫描电镜观察等验证手段均证实所得到的确实是无藻萍。由茎尖培养获得的无藻萍经九年有氮培养和由切除雌雄混养的大孢子果顶端囊群盖与漏斗状膜而获得的无藻萍经两年多有氮培养,生长正常,也证明由这两种生物技术取得的无藻萍是可靠的,不像由化学或物理方法取得的无藻萍反复不定。本文还讨论了无藻萍的理论价值和实践意义。Abstract: The Anabaena-free azolla was successfully acquired by using tissue culture from the tip of the azolla frond or eliminating Anabaena cells from inner portion of indusium cap and apical membrane of megasporo-carps. The identifications through sectioning, cavity liquid examination, N-free medium cultivation, Nase determination and scanning electromicro-scopic observation proved that the obtained azolla fronds were really Anabaena-free azolla. The Anabaena-free azolla acquired by tip culture and that acquired by removing the indusium cap and apical membrane were continuously grown in nitorgenous culture media for 9 and 2 years respectively. After that they kept making a normal growth. The fact illustrated that the above-mentioned methods to acquire Anabaena-free azolla were reliable. However, the Anabaena-free azolla obtained by chemical or physical means was unstable.The theoretical value and practical significance of Anabaena-free azo-lla were also discussed in this paper.
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[3] 郑德英等,1980,茎尖培养获得无藻萍。福建农业科技4:31~32
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