凤尾菇栽培中固氮问题的探讨
EXPLORATION ON NITROGEN FIXATION IN THE CULTIVATION OF PLEUROTUS SAJOR——CAJU
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摘要: 本研究结果表明:①虽然凤尾菇在阿须贝培养基上尚能生长,但在多种灭菌过的培养基上都测不出其固氮酶活性,15N检测中6个重复也测不出凤尾菇能利用15N。这就可以证明它本身是不具有固氮能力的。②同样接种凤尾菇,由于开水烫的培养料有固氮菌存在,比用高压灭菌处理的培养料的固氮酶活性高出19~62倍,当凤尾菇与固氮菌一块生长时,所固定的15N数量比单有固氮菌时会增加10多倍。这些事实证明了凤尾菇栽培中氮素的增加也不是无中生有,而是来自料中各种固氮菌共同固氮的结果。Abstract: The results of this investigation showed that 1) although Pleurotus sajor-caju could grow on Ashby medium, it had no N-fixing activity in various sterilized media. The determination by using the I5N tracing method in 6 replications also made clear that Pleurotus sajor-caju was unable to utilize atmospheric 15N. These facts demonstrated that Pleurotus sajor-caju itself had no nitrogenase activity; 2) after inoculating Pleurotus sajor-caju, the nitrogenase activity war. 19-62 times higher in the medium treated by boiling water than in the medium treated by autoclave. That is because more N-fixing microorganisms were found in the medium treated by boiling water. The pleurotus sajor-caju which was inoculated in the medium sterilized by autoclave failed to fix 15N, whereas the one in the medium treated by boiling water might fix 15N. The N-fixing microorganisms which were isolated from culture medium possessed various levels of nitrogen fixation. These facts illustrated that the increase of nitrogen amount in the cultivation of Pleurotus sajor-caju might be attributed to the associative nitrogen fixation coordinated by different kinds of N-fixing microorganisms in the medium; 3) although the 15N amount fixet in the medium where Pleurotus sajor-caju was grown together with N-fixing microorgainisms was more than ten times higher than that fixed in the medium where only N-fixing microorganisms were grown, it was not always the case that the combining growth of the Pleurotus sajor-caju with N-fixing microorganisms isolated from medium might increase the N-fixing activity. The results received from the experiment showed that some combinations increased N-fixing activity significantly, but some combinations did not.
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