摘要:
用四个家系共97个13日龄的鸭胚进行活组织培养,测定培养液中的碱性磷酸酶活性。结果表明,该酶的活性强度与其半同胞姐妹鸭的开产日龄呈负相关(r=-0.7131);与240及300日龄的产蛋数呈正相关,相关系数分别为0.8611和0.9403。当该酶活性在4～7金氏单位时,其半同胞姐妹鸭的开产日龄,比活性为8～12金氏单位的迟16～17天;240和300日龄的产蛋数,分别少12～25和15～25个,差异显著。根据鸭胚组织培养液中碱性磷酸酶的活性,早期评定蛋鸭不同家系的种用价值,比用常规法选种可提早半年以上。
Abstract:
In this experiment, the method of embryonic tissue culture and the biochemical method have been used to determine the activity of AKP in the tissue culture fluid which is from 97 duck embryos, aged 13 days, belonging to 4 families; and the productive traits of the half-sib female ducks have also been recorded and studied.The results show: the active intensity of AKP in the duck embryo tissue culture fluid is negatively correlated with the days of sex-maturity of the half-sib female ducks(r=-0.713), but it is positively correlated with the laying number of the ducks aged 240 and 300 days.The correlation coefficients are 0.866 and 0.940 respectively. When the activity of AKP in the duck embryo tissue culture fluid is at 4-7 King units, the sex-maturity of the half-nib female ducks is at the age of 137-143 days, while at 8-12 King units, the sex-maturity is at the age of 121-126 days, 16-17 days earlier than the former. The different is significant(p0.05). As the active intensity rises, the laying number of its half-nib female ducks aged 240 and 300 increases; the difference among the families are highly significant(p0.01).Therefore, according to the activity of AKP in duck embryo tissue culture fluid, we evaluate the stud egg-laying ducks among different families in the early stage. It can be over half a year earlier than if the traditional method is adopted.
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