摘要:
1975年采得病死茶毛虫尸体。该病原被鉴定为茶毛虫核型多角体病毒(简称EPNPV),在细胞核内增殖,形成多角体,病毒束为短柱状,核衣壳为杆状,多粒包埋。核酸构型为单分子双链环状超螺旋DNA,分子量96～97×106道尔顿。病毒核酸内切酶图谱及蛋白结构多肽图谱分析都具有其特异性。用它的DNA探针与其他NPV-DNA杂交,鉴定出EPNPV与其他NPV-DNA的不同同源性程度。该毒株毒力生物测定,对三至五龄幼虫LG50为1.18～1.58×105多角体/毫升。病毒增殖采用茶园治虫回收、迁集定园增殖、室内催孵催育然后罩控等简易方法,大量增殖病毒,试制了病毒粉剂、乳剂。1976年以来,于不同龄期、季节、气候、海拔、地区以3～6×107多角体/毫升在茶园进行试验与示范共1400多亩,杀虫率为71～97%,并观察到在茶园使用有其流行传播及持续作用。1985～1986年在福州、寿宁等地茶园推广4000多亩,杀虫率为82.9%以上,年亩有10～50元以上的经济效益(挽回损失)。对喂饲茶园优势种天敌瓢虫、蜘蛛试验无害。对实验动物的安全性试验表明,对人畜安全无害,有明显的社会效益。
Abstract:
Pathogen was separated from the bodies of infected Euproctis pseudo-cor.spersa larvae and was identified as Euproctis pseudoconspersa nuclear poly-hedrosis virus (EPNPV). The rod-shaped virus belongs to the multi -granule inclusion type. Its DNA is of the configuration of monomolecu-lar double-strand superhelix,with a molecular weight of 9.6-9.7?107 Dolton.According to the results of bioassey on Euproctis pseudoconspersa larvae (3-5 instar), the LC50 of EPNPV was 1.18-1.58?105 PIB/ml. Large amounts of the virus was obtained by following methods: 1. applying the virus to tea bush and then collecting infected larvae; 2. transferring the infected larvae to a tea plot for reproduction; 3. reproducing on laboratory conditions.Formulations of powder and emulsion were made for field experiment. According to the field experiment conducted since 1976 on different instared larvae under various ecological conditions, a control rate of 71 -97% was observed in a dose of 3-6?107 PIB/ml, with some persistent effect.During 1985 and 1986, about 4000 mu of tea plantation was treated with EPNPV. The control rate was 82-90% or more. Significant economic benefits were reaped. EPNPV is safe to humans and animals and not toxic to such main predators as spiders and ladybugs.
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